IL1A ELISA Kit (Interleukin 1 alpha)

Details for Product IL1A ELISA Kit No. ABIN625017, Supplier: Log in to see
Antigen
  • Il-1
  • Il-1a
  • IL-1 alpha
  • IL-1A
  • IL1
  • IL1-ALPHA
  • IL1F1
  • IL-1alpha
  • L1A
  • interleukin 1 complex
  • interleukin 1 alpha
  • interleukin 1, alpha
  • Il1
  • Il1a
  • IL1A
Alternatives
Human IL1A ELISA Kit
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
53
44
33
12
12
11
10
8
7
4
3
2
2
1
Method Type
Sandwich ELISA
Detection Range
0.5-300 pg/mL
Minimum Detection Limit
0.5 pg/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human IL-1 alpha (IL-1 F1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity 0.5 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Alternative Name IL-1 alpha (IL1A ELISA Kit Abstract)
Background Gene Names: IL1A IL1F1
Protein names: Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
Gene ID 3552
UniProt P01583
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, Autophagy
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Supplier Images
ELISA image for Interleukin 1 alpha (IL1A) ELISA Kit (ABIN625017) Interleukin 1 alpha (IL1A) ELISA Kit
Product cited in: Park, Cheon: "Malignant small bowel obstruction: the last frontier for gastrointestinal stenting." in: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1136-7, 2012 (PubMed).

Goktas, Coskun, Bicik, Horuz, Unsal, Serteser, Albayrak, Sarıca: "Evaluating ESWL-induced renal injury based on urinary TNF-α, IL-1α, and IL-6 levels." in: Urological research, Vol. 40, Issue 5, pp. 569-73, 2012 (PubMed).

Cuchacovich, Hagan, Khan, Richert, Espinoza: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." in: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, 2011 (PubMed).

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