bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)

Details for Product No. ABIN1339644, Supplier: Log in to see
Protein Species
Human
Species of Lysate
Human
Application
Immunoprecipitation (IP), Western Blotting (WB)
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Purpose Whole cell lysate
Specificity Non-denatured
Characteristics b mMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
Used Tissue: Bone Marrow
Background Full Gene Name:
Synonyms:
Application Notes Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Comment

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions For Research Use only
Format Liquid
Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -80 °C
Storage Comment Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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 image for bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured) (ABIN1339644) bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
Product cited in: Chang, Shih, Tseng, Hsieh, Lee, Hwang: "Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, 2006 (PubMed).