SMAD3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
SMAD3
IP, WB
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Human
-
- Target See all SMAD3 products
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
SMAD3 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-SMAD3 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
-
- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-SMAD3 antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- Alternative Name
- SMAD3 (SMAD3 Products)
- Synonyms
- HSPC193 Lysate, HsT17436 Lysate, JV15-2 Lysate, LDS1C Lysate, LDS3 Lysate, MADH3 Lysate, madh3 Lysate, madh3a Lysate, smad3 Lysate, wu:fa99e03 Lysate, XSmad3 Lysate, XenMLP Lysate, Xmad3 Lysate, madh3-A Lysate, Madh3 Lysate, AU022421 Lysate, Smad 3 Lysate, mad3 Lysate, madh3b Lysate, zgc:92234 Lysate, SMAD family member 3 Lysate, SMAD family member 3a Lysate, SMAD family member 3 L homeolog Lysate, SMAD family member 3b Lysate, SMAD3 Lysate, smad3a Lysate, smad3.L Lysate, Smad3 Lysate, smad3b Lysate
- Background
-
Full Gene Name: SMAD family member 3
Synonyms: DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396 - Gene ID
- 4088
- NCBI Accession
- NP_005893, NM_005902
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