MCF-7 nuclear extract (H2O2 treated)
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- Species of Lysate
- Human Cells
- Characteristics
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Origin: human breast carcinoma
- Components
- 2 x 100 μg of MCF-7 nuclear extract (H2O2 treated) at 2.50 μg/μl
- Lysed Cells
- MCF7 Cells
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Nuclear Extract
- Lysate Treatment
- H2O2 treated
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
Hep2 Whole Cell LysateWB Whole Cell Lysate Cell Lysate HepG2 Cells Reactivity: Human
NIH/3T3 Whole Cell Lysate (PDGF Stimulated)WB Whole Cell Lysate Cell Lysate 3T3 Cells PDGF Stimulated Reactivity: Mouse
DYKDDDDK Positive Control LysatePC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Tag
A431 Whole Cell Lysate (EGF Stimulated)WB Whole Cell Lysate Cell Lysate A431 Cells EGF treated Reactivity: Human
CD177 (human): 293T LysateCD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
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- Restrictions
- For Research Use only
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- Concentration
- 2.50 μg/μL
- Buffer
- The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with 0.2 mM H2O2. The Lysis Buffer consists of 20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT. The protein content has been determined by a Bradford-based assay.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
- Storage
- -80 °C
- Storage Comment
- To ensure stability, extracts should be stored at -80°C.
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