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|Application / Reactivity||Human||Mouse (Murine)|
|Western Blotting (WB)||380 Antibodies||186 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies|
|Immunoprecipitation (IP)||85 Antibodies||56 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||85 Antibodies||49 Antibodies|
|Immunohistochemistry (IHC)||246 Antibodies||160 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||105 Antibodies||94 Antibodies|
|Immunofluorescence (IF)||83 Antibodies||39 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies||4 Antibodies|
|Antigen||Activating Transcription Factor 2 (ATF2) Antibodies|
|Epitope||pSer44, pSer62 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This ATF2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|5 references available|
|Supplier||Log in to see|
Product Details anti-ATF2 AntibodyTarget Details ATF2 Application Details Handling References for anti-ATF2 Antibody (ABIN196662) Images
|Specificity||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. ATF-2 (phospho-Ser62 or 44) antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 62 or 44.|
|Immunogen||The antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 62 or 44 (N-D-SP-V-I).|
Target Details ATF2Product Details anti-ATF2 Antibody Application Details Handling References for anti-ATF2 Antibody (ABIN196662) Images back to top
|Alternative Name||ATF2 (ATF2 Antibody Abstract)|
|Background||ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.Synonyms: ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2|
|Research Area||Immunology, Innate Immunity, Transcription Factors|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling|
Application DetailsProduct Details anti-ATF2 Antibody Target Details ATF2 Handling References for anti-ATF2 Antibody (ABIN196662) Images back to top
Suitable for use in Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ATF2 Antibody Target Details ATF2 Application Details References for anti-ATF2 Antibody (ABIN196662) Images back to top
|Buffer||Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at -20 °C.|
References for anti-ATF2 Antibody (ABIN196662)Product Details anti-ATF2 Antibody Target Details ATF2 Application Details Handling Images back to top
Sevilla, Santos, Vega et al.: "Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27458-65, 2004 (PubMed).
Gupta, Campbell, Dérijard et al.: "Transcription factor ATF2 regulation by the JNK signal transduction pathway." in: Science (New York, N.Y.), Vol. 267, Issue 5196, pp. 389-93, 1995 (PubMed).
van Dam, Wilhelm, Herr et al.: "ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents." in: The EMBO journal, Vol. 14, Issue 8, pp. 1798-811, 1995 (PubMed).
Sakurai, Maekawa, Sudo et al.: "Phosphorylation of cAMP response element-binding protein, CRE-BP1, by cAMP-dependent protein kinase and protein kinase C." in: Biochemical and biophysical research communications, Vol. 181, Issue 2, pp. 629-35, 1992 (PubMed).
Abdel-Hafiz, Heasley, Kyriakis et al.: "Activating transcription factor-2 DNA-binding activity is stimulated by phosphorylation catalyzed by p42 and p54 microtubule-associated protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 6, Issue 12, pp. 2079-89, 1993 (PubMed).
ImagesProduct Details anti-ATF2 Antibody Target Details ATF2 Application Details Handling References for anti-ATF2 Antibody (ABIN196662) back to top