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anti-Rat (Rattus) ATF2 antibody for Immunohistochemistry (Frozen Sections)

Recommended ATF2 Antibody (supplied by: Log in to see )

Antigen
Activating Transcription Factor 2 (ATF2) Antibodies
  • Atf-2
  • ATF1
  • ATF2
  • atf2-A
  • atf2l
  • CG3749
  • CG12850
  • CG30420
  • CHN1
  • CRE-BP
  • CRE-BP1
  • Creb2
  • CREB2
  • D18875
  • D130078H02Rik
  • dATF-2
  • DmelCG30420
  • HB16
  • HFB1-GDNF
  • HSCR3
  • mXBP
  • Tg(Gzma-Klra1)7Wum
  • TREB7
Epitope
pSer490, pSer498
73
47
41
34
33
33
32
32
28
26
26
25
18
18
13
13
12
11
10
9
8
8
8
7
7
7
6
6
6
5
5
5
5
5
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
603
336
307
20
9
6
5
4
2
2
2
1
1
1
1
Host
Rabbit
536
51
15
4
Clonality
Polyclonal
Conjugate
This ATF2 antibody is un-conjugated
16
15
15
10
10
9
9
9
9
9
9
9
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
414
259
156
100
97
94
86
22
21
12
10
6
3
2
1
1
1
Supplier
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Catalog No. ABIN372591
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000884117 ABIN3043791 IHC (fro) IHC (p) WB Rabbit IgG AA 93-450 Log in to see Polyclonal
0.000884117 ABIN3029845 IHC (fro) IHC (p) WB Rabbit IgG AA 93-450 Log in to see Polyclonal

Top referenced anti-ATF2 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-ATF2 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 62 Antibodies 157 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies 10 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 47 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 78 Antibodies
Immunohistochemistry (IHC) 167 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 62 Antibodies
Western Blotting (WB) 184 Antibodies
Dot Blot (DB) 23 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 2 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 21 Antibodies

General

Antigen Activating Transcription Factor 2 (ATF2) Antibodies
Epitope pSer490, pSer498
(73), (47), (41), (34), (33), (33), (32), (32), (28), (26), (26), (25), (18), (18), (13), (13), (12), (11), (10), (9), (8), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(603), (336), (307), (20), (9), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(536), (51), (15), (4)
Clonality
Polyclonal
Conjugate This ATF2 antibody is un-conjugated
(16), (15), (15), (10), (10), (9), (9), (9), (9), (9), (9), (9), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(414), (259), (156), (100), (97), (94), (86), (22), (21), (12), (10), (6), (3), (2), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-ATF2 Antibody

Target Details ATF2 Application Details Handling References for anti-ATF2 Antibody (ABIN372591) Images
Specificity Specific for ~74k ATF2 protein phosphorylated at Ser490 and 498. The antibody also recognizes the phosphorylated ~54k splice form of ATF2.
Cross-Reactivity (Details) Species reactivity (expected):Rat.
Species reactivity (tested):Human.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser490,498 of human ATF2.
Isotype IgG

Target Details ATF2

Product Details anti-ATF2 Antibody Application Details Handling References for anti-ATF2 Antibody (ABIN372591) Images back to top
Antigen
Alternative Name ATF2 (ATF2 Antibody Abstract)
Background The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003, Morton et al., 2004). Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000), Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004).Synonyms: ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2
Gene ID 1386
NCBI Accession NP_001871
UniProt P15336
Research Area Immunology, Innate Immunity, Transcription Factors
Pathways MAPK Signaling, RTK Signaling, TCR Signaling

Application Details

Product Details anti-ATF2 Antibody Target Details ATF2 Handling References for anti-ATF2 Antibody (ABIN372591) Images back to top
Application Notes Western Blot: 1/1000. Immunohistochemistry on Frozen Sectios: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ATF2 Antibody Target Details ATF2 Application Details References for anti-ATF2 Antibody (ABIN372591) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-ATF2 Antibody (ABIN372591)

Product Details anti-ATF2 Antibody Target Details ATF2 Application Details Handling Images back to top
Product cited in:

Muñoz, Kawahara, Yannone: "An autonomous chromatin/DNA-PK mechanism for localized DNA damage signaling in mammalian cells." in: Nucleic acids research, Vol. 41, Issue 5, pp. 2894-906, 2013

Background publications

Laifenfeld, Karry, Grauer, Klein, Ben-Shachar: "ATF2, a member of the CREB/ATF family of transcription factors, in chronic stress and consequent to antidepressant treatment: animal models and human post-mortem brains." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 29, Issue 3, pp. 589-97, 2004

Morton, Davis, Cohen: "Signalling pathways involved in multisite phosphorylation of the transcription factor ATF-2." in: FEBS letters, Vol. 572, Issue 1-3, pp. 177-83, 2004

Ivanov, Bhoumik, Ronai: "Death receptors and melanoma resistance to apoptosis." in: Oncogene, Vol. 22, Issue 20, pp. 3152-61, 2003

Fuchs, Tappin, Ronai: "Stability of the ATF2 transcription factor is regulated by phosphorylation and dephosphorylation." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12560-4, 2000

Karin, Hunter: "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus." in: Current biology : CB, Vol. 5, Issue 7, pp. 747-57, 1995

Maekawa, Sakura, Kanei-Ishii, Sudo, Yoshimura, Fujisawa, Yoshida, Ishii: "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain." in: The EMBO journal, Vol. 8, Issue 7, pp. 2023-8, 1989

Images

Product Details anti-ATF2 Antibody Target Details ATF2 Application Details Handling References for anti-ATF2 Antibody (ABIN372591) back to top
Supplier Images
 image for anti-ATF2 antibody (Activating Transcription Factor 2) (pSer490) (ABIN372591) anti-Activating Transcription Factor 2 (ATF2) (pSer490), (pSer498) antibody