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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||59 Antibodies||153 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies||10 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||43 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||83 Antibodies|
|Immunohistochemistry (IHC)||155 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||38 Antibodies|
|Immunoprecipitation (IP)||53 Antibodies|
|Western Blotting (WB)||172 Antibodies|
|Dot Blot (DB)||23 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Antigen||Activating Transcription Factor 2 (ATF2) Antibodies|
|Epitope||pSer490, pSer498 Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This ATF2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-ATF2 AntibodyTarget Details ATF2 Application Details Handling References for anti-ATF2 Antibody (ABIN372591) Images
|Specificity||Specific for ~74k ATF2 protein phosphorylated at Ser490 and 498. The antibody also recognizes the phosphorylated ~54k splice form of ATF2.|
Species reactivity (expected):Rat.
Species reactivity (tested):Human.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser490,498 of human ATF2.|
Target Details ATF2Product Details anti-ATF2 Antibody Application Details Handling References for anti-ATF2 Antibody (ABIN372591) Images back to top
|Alternative Name||ATF2 (ATF2 Antibody Abstract)|
|Background||The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003, Morton et al., 2004). Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000), Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004).Synonyms: ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2|
|Research Area||Immunology, Innate Immunity, Transcription Factors|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling|
Application DetailsProduct Details anti-ATF2 Antibody Target Details ATF2 Handling References for anti-ATF2 Antibody (ABIN372591) Images back to top
Western Blot: 1/1000. Immunohistochemistry on Frozen Sectios: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ATF2 Antibody Target Details ATF2 Application Details References for anti-ATF2 Antibody (ABIN372591) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-ATF2 Antibody (ABIN372591)Product Details anti-ATF2 Antibody Target Details ATF2 Application Details Handling Images back to top
|Product cited in:||
Muñoz, Kawahara, Yannone: "An autonomous chromatin/DNA-PK mechanism for localized DNA damage signaling in mammalian cells." in: Nucleic acids research, Vol. 41, Issue 5, pp. 2894-906, 2013 (PubMed).
Morton, Davis, Cohen: "Signalling pathways involved in multisite phosphorylation of the transcription factor ATF-2." in: FEBS letters, Vol. 572, Issue 1-3, pp. 177-83, 2004 (PubMed).
Laifenfeld, Karry, Grauer et al.: "ATF2, a member of the CREB/ATF family of transcription factors, in chronic stress and consequent to antidepressant treatment: animal models and human post-mortem brains." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 29, Issue 3, pp. 589-97, 2004 (PubMed).
Ivanov, Bhoumik, Ronai: "Death receptors and melanoma resistance to apoptosis." in: Oncogene, Vol. 22, Issue 20, pp. 3152-61, 2003 (PubMed).
Fuchs, Tappin, Ronai: "Stability of the ATF2 transcription factor is regulated by phosphorylation and dephosphorylation." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12560-4, 2000 (PubMed).
Karin, Hunter: "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus." in: Current biology : CB, Vol. 5, Issue 7, pp. 747-57, 1995 (PubMed).
Maekawa, Sakura, Kanei-Ishii et al.: "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain." in: The EMBO journal, Vol. 8, Issue 7, pp. 2023-8, 1989 (PubMed).
ImagesProduct Details anti-ATF2 Antibody Target Details ATF2 Application Details Handling References for anti-ATF2 Antibody (ABIN372591) back to top