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Mammalian BCL2 ELISA Kit

Recommended BCL2 Product (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) ELISA Kits
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Reactivity
Mammalian
Alternatives
Kits with alternative reactivity to:
12
9
9
3
1
1
1
1
1
Application
ELISA
Supplier
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Supplier Product No.
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Catalog No. ABIN2869683
$ 1,205.25
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012134541 ABIN2869626 Log in to see

Similar BCL2 ELISA Kits

Application / Reactivity Mammalian
ELISA 2 ELISA Kits

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) ELISA Kits
Reactivity Mammalian
Kits with alternative reactivity to:
(12), (9), (9), (3), (1), (1), (1), (1), (1)
Application ELISA
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Product Details BCL2 ELISA Kit

Target details Application Details Handling Images
Detection Method Colorimetric
Characteristics ELISA kit for the detection of Bcl2 in the research laboratory
Alternative Names: Bcl-2 ELISA kit, Apoptosis regulator Bcl-2 ELISA kit
Purification Cell supernatant, Protein A purified

Target details

Product Details BCL2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Bcl2 (BCL2 ELISA Kit Abstract)
Background Bcl2 initiates a new gene family involved in the regulation of cell death and survival without affecting cell proliferation. This precise regulation and maintenance of balance between cell proliferation and cell death in multicellular organisms is critical for tissue homeostasis. Bcl2, encoded by a protooncogene, is an intracellular membrane-associated protein that functions to block programmed cell death. Expression of Bcl2 has been reported in a wide range of hematopoietic cells, non-neoplastic epithelia and epithelial malignancies. Bcl2 levels are low or undetectable in endothelial cells. The Bcl2 gene has a unique function among mammalian oncogenes as a negative regulator of apoptosis. It was first discovered because of its involvement in chromosomal translocations commonly found in lymphomas. Bcl2 is furthermore associated with stem cells committed to differentiation and morphogenesis.
Synonyms: Bcl-2 ELISA kit, Apoptosis regulator Bcl-2 ELISA kit.
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details BCL2 ELISA Kit Target details Handling Images back to top
Application Notes Optimal conditions to be determined by end-user
Assay Procedure An antihuman Bcl2 coating antibody is adsorbed onto microwells. Human Bcl2 present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman Bcl2 antibody is added and binds to human Bcl2 captured by the first antibody. Following incubation unbound biotinconjugated antihuman Bcl2 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman Bcl2 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Bcl2 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human Bcl2 standard dilutions and human Bcl2 sample concentration determined.
Restrictions For Research Use only

Handling

Product Details BCL2 ELISA Kit Target details Application Details Images back to top
Buffer 0,01 M sodiumphosphate, 0,15 M sodium chloride pH 7,2
Storage 4 °C
Storage Comment Store at 2-8 °C.