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|Antigen||B-Cell CLL/lymphoma 2 (BCL2) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
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Product Details BCL2 ELISA KitTarget details Application Details Handling Images
ELISA kit for the detection of Bcl2 in the research laboratory
Alternative Names: Bcl-2 ELISA kit, Apoptosis regulator Bcl-2 ELISA kit
|Purification||Cell supernatant, Protein A purified|
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|Alternative Name||Bcl2 (BCL2 ELISA Kit Abstract)|
Bcl2 initiates a new gene family involved in the regulation of cell death and survival without affecting cell proliferation. This precise regulation and maintenance of balance between cell proliferation and cell death in multicellular organisms is critical for tissue homeostasis. Bcl2, encoded by a protooncogene, is an intracellular membrane-associated protein that functions to block programmed cell death. Expression of Bcl2 has been reported in a wide range of hematopoietic cells, non-neoplastic epithelia and epithelial malignancies. Bcl2 levels are low or undetectable in endothelial cells. The Bcl2 gene has a unique function among mammalian oncogenes as a negative regulator of apoptosis. It was first discovered because of its involvement in chromosomal translocations commonly found in lymphomas. Bcl2 is furthermore associated with stem cells committed to differentiation and morphogenesis.
Synonyms: Bcl-2 ELISA kit, Apoptosis regulator Bcl-2 ELISA kit.
|Research Area||Cancer, Apoptosis/Necrosis, Metabolism|
|Pathways||MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling|
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|Application Notes||Optimal conditions to be determined by end-user|
|Assay Procedure||An antihuman Bcl2 coating antibody is adsorbed onto microwells. Human Bcl2 present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman Bcl2 antibody is added and binds to human Bcl2 captured by the first antibody. Following incubation unbound biotinconjugated antihuman Bcl2 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman Bcl2 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Bcl2 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human Bcl2 standard dilutions and human Bcl2 sample concentration determined.|
|Restrictions||For Research Use only|
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|Buffer||0,01 M sodiumphosphate, 0,15 M sodium chloride pH 7,2|
|Storage Comment||Store at 2-8 °C.|