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anti-Mouse (Murine) ELK1 antibody for Intracellular Staining

Recommended ELK1 Antibody (supplied by: Log in to see )

Antigen
ELK1, Member of ETS Oncogene Family (ELK1) Antibodies
  • BEC2
  • Elk-1
  • ELK1
  • Kv12.3
Epitope
pSer383
72
33
33
26
12
8
7
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
347
172
165
11
4
4
2
2
2
2
1
Host
Mouse
278
73
Clonality
Monoclonal
Conjugate
This ELK1 antibody is conjugated to Alexa Fluor 647
7
7
7
6
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Intracellular Staining (ICS)
237
136
125
41
40
34
33
7
4
2
2
1
1
Supplier
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Catalog No. ABIN1177054
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086434494 ABIN1177055 ICS PE Mouse IgG1, kappa pSer383 Log in to see M21-1721 4
0.00086434494 ABIN1177056 ICS Alexa Fluor 488 Mouse IgG1, kappa pSer383 Log in to see M21-1721 5

Top referenced anti-ELK1 antibody for Intracellular Staining

Similar anti-ELK1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Western Blotting (WB) 235 Antibodies 94 Antibodies 96 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 42 Antibodies 32 Antibodies 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies 30 Antibodies 25 Antibodies
Immunohistochemistry (IHC) 138 Antibodies 87 Antibodies 78 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies 34 Antibodies

General

Antigen ELK1, Member of ETS Oncogene Family (ELK1) Antibodies
Epitope pSer383
(72), (33), (33), (26), (12), (8), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(347), (172), (165), (11), (4), (4), (2), (2), (2), (2), (1)
Host Mouse
(278), (73)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ELK1 antibody is conjugated to Alexa Fluor 647
(7), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Intracellular Staining (ICS)
(237), (136), (125), (41), (40), (34), (33), (7), (4), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-ELK1 Antibody

Target Details ELK1 Application Details Handling References for anti-ELK1 Antibody (ABIN1177054) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Elk-1 Peptide
Clone M21-1721
Isotype IgG1, kappa

Target Details ELK1

Product Details anti-ELK1 Antibody Application Details Handling References for anti-ELK1 Antibody (ABIN1177054) Images back to top
Antigen
Alternative Name Elk-1 (ELK1 Antibody Abstract)
Background Elk-1 is a 62-kDa member of the Ets oncogene family of transcription factors and a member of the subfamily of ternary complex factors (TCF). Ets proteins mediate a variety of gene activities in response to serum and growth factors. Proteins in the TCF subfamily form a ternary complex by binding to the Serum Response Element (SRE) in conjunction with a dimer of Serum Response Factors (SRF). Elk-1 is phosphorylated by mitogen-activated protein (MAP) kinase pathways in vivo at a cluster of S/T motifs at its carboxy-terminus. Phosphorylation at these sites, particularly at serine 383 (S383), is critical for Elk-1 transcriptional activation. Studies have shown that Elk-1 is a direct target of activated MAP kinase, and that it is also a target of the stress-activated kinase SAPK/JNK. The M21-1721 monoclonal antibody recognizes the phosphorylated S383 of activated Elk-1. The orthologous phosphorylation sites of mouse and rat Elk-1 are S384 and S382, respectively.
Synonyms: ELK1
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ELK1 Antibody Target Details ELK1 Handling References for anti-ELK1 Antibody (ABIN1177054) Images back to top
Application Notes This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-ELK1 Antibody Target Details ELK1 Application Details References for anti-ELK1 Antibody (ABIN1177054) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-ELK1 Antibody (ABIN1177054)

Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling Images back to top
Product cited in:

Bardwell, Abdollahi, Bardwell: "Docking sites on mitogen-activated protein kinase (MAPK) kinases, MAPK phosphatases and the Elk-1 transcription factor compete for MAPK binding and are crucial for enzymic activity." in: The Biochemical journal, Vol. 370, Issue Pt 3, pp. 1077-85, 2003

Espanel, Wälchli, Rückle, Harrenga, Huguenin-Reggiani, Hooft van Huijsduijnen: "Mapping of synergistic components of weakly interacting protein-protein motifs using arrays of paired peptides." in: The Journal of biological chemistry, Vol. 278, Issue 17, pp. 15162-7, 2003

Tian, Karin: "Stimulation of Elk1 transcriptional activity by mitogen-activated protein kinases is negatively regulated by protein phosphatase 2B (calcineurin)." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 15173-80, 1999

Sugimoto, Stewart, Guan: "The calcium/calmodulin-dependent protein phosphatase calcineurin is the major Elk-1 phosphatase." in: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29415-8, 1997

Cavigelli, Dolfi, Claret, Karin: "Induction of c-fos expression through JNK-mediated TCF/Elk-1 phosphorylation." in: The EMBO journal, Vol. 14, Issue 23, pp. 5957-64, 1996