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anti-Dog (Canine) ERK2 antibody for Immunofluorescence

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • 9030612K14Rik
  • AA407128
  • ATMPK1
  • ATMPK2
  • AU018647
  • C78273
  • erk
  • ERK
  • ERK2
  • Erk2
  • erk2
  • ert1
  • ERT1
  • F14N23.9
  • F14N23_9
  • mapk
  • mapk1
  • MAPK1
  • mapk1a
  • mapk1b
  • MAPK2
  • mapk2
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • mitogen-activated protein kinase homolog 2
  • mpk1
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • P42MAPK
  • Prkm1
  • prkm1
  • PRKM1
  • PRKM2
  • prkm2
  • T30E16.13
  • T30E16_13
  • Xp42
  • xp42
  • zERK2
Epitope
AA 219-358
62
25
15
9
8
8
7
5
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
314
98
68
33
16
7
4
3
3
3
2
2
1
1
1
1
1
Host
Mouse
160
157
16
3
2
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
8
8
7
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
298
140
82
77
65
50
49
25
17
10
5
3
2
1
1
1
1
1
1
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Catalog No. ABIN967747
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32106254 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
0.32106254 ABIN2452330 IF IP WB Mouse IgG2a Log in to see 7C9
0.32106254 ABIN2452333 IF IP WB Mouse IgG1 Log in to see 7E7
0.0010843406 ABIN2452608 IF IP IHC WB Mouse IgG1 Log in to see 4C2

Top referenced anti-ERK2 antibody for Immunofluorescence

Similar anti-ERK2 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine) Rat (Rattus) Chicken Frog
Western Blotting (WB) 279 Antibodies 17 Antibodies 89 Antibodies 64 Antibodies 4 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 13 Antibodies 39 Antibodies 18 Antibodies 22 Antibodies 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope AA 219-358
(62), (25), (15), (9), (8), (8), (7), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(314), (98), (68), (33), (16), (7), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(160), (157), (16), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(8), (8), (7), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(298), (140), (82), (77), (65), (50), (49), (25), (17), (10), (5), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN967747) Images
Cross-Reactivity Human, Dog (Canine), Mouse (Murine), Chicken, Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat ERK2 aa. 219-358
Clone 33-ERK2
Isotype IgG2b

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 Antibody (ABIN967747) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) are activated after cell stimulation by a wide variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. Structural analysis of ERK2 indicates that phosphorylation induces a conformational change that exposes the active site for substrate binding. Myriad proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK2 is the 42kDa member of the ERK family and is highly homologous to ERK1.
Molecular Weight 42 kDa
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 Antibody (ABIN967747) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 Antibody (ABIN967747) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-ERK2 Antibody (ABIN967747)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Product cited in:

Kim, Ju, Oh, Yoon, Song, Kim, Yoo, Bang, Kang, Chun: "ERK-1/2 and p38 kinase oppositely regulate nitric oxide-induced apoptosis of chondrocytes in association with p53, caspase-3, and differentiation status." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1332-9, 2002

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, 2000

Lehmann, Janda, Pierreux, Rytömaa, Schulze, McMahon, Hill, Beug, Downward: "Raf induces TGFbeta production while blocking its apoptotic but not invasive responses: a mechanism leading to increased malignancy in epithelial cells." in: Genes & development, Vol. 14, Issue 20, pp. 2610-22, 2000

Liu, Tsai, Aird: "Egr-1 gene is induced by the systemic administration of the vascular endothelial growth factor and the epidermal growth factor." in: Blood, Vol. 96, Issue 5, pp. 1772-81, 2000

Visconti, Gadina, Chiariello, Chen, Stancato, Gutkind, OShea: "Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity." in: Blood, Vol. 96, Issue 5, pp. 1844-52, 2000

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN967747) back to top
Supplier Images
Western Blotting (WB) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (AA 219-358) (ABIN967747) Western blot analysis of ERK2 on rat pituitary lysate. Lane 1: 1:5000, lane 2: 1:1000...
Immunofluorescence (IF) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (AA 219-358) (ABIN967747) Immunofluorescence staining of MCF7 cells.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (AA 219-358) (ABIN967747) ERK 2 (clone 33) staining on rat brain. Formalin fixed paraffin section with citrate ...