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anti-Mouse (Murine) ERK2 antibody for Immunoprecipitation

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • 9030612K14Rik
  • AA407128
  • ATMPK1
  • ATMPK2
  • AU018647
  • C78273
  • erk
  • ERK
  • ERK2
  • Erk2
  • erk2
  • ert1
  • ERT1
  • F14N23.9
  • F14N23_9
  • mapk
  • mapk1
  • MAPK1
  • mapk1a
  • mapk1b
  • MAPK2
  • mapk2
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • mitogen-activated protein kinase homolog 2
  • mpk1
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • P42MAPK
  • Prkm1
  • prkm1
  • PRKM1
  • PRKM2
  • prkm2
  • T30E16.13
  • T30E16_13
  • Xp42
  • xp42
  • zERK2
Epitope
C-Term
60
25
15
9
8
8
7
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
322
105
70
36
16
7
5
3
3
2
2
2
1
1
1
1
Host
Mouse
163
160
16
3
2
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
8
8
7
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
301
144
89
84
68
53
50
26
19
9
5
3
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN126832
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000895667 ABIN967397 IP WB Mouse IgG1, kappa Log in to see G263-7 1
0.000895667 ABIN967747 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
0.000895667 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
0.000895667 ABIN302975 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.000895667 ABIN492351 EIA IP WB Biotin Mouse IgG1 C-Term Log in to see 6G11
0.000895667 ABIN2469740 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.000895667 ABIN601266 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.000895667 ABIN1736732 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.000895667 ABIN210913 IP WB Rabbit IgG Log in to see Polyclonal
0.000895667 ABIN1839821 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.000895667 ABIN2306383 IP ELISA WB Mouse IgG2a Log in to see 0-T-86
0.000895667 ABIN2306385 IP WB Rabbit IgG Log in to see Polyclonal
0.000895667 ABIN2261950 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.000895667 ABIN4270497 IP WB Rabbit Log in to see T8
0.000895667 ABIN2306384 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.000895667 ABIN292900 IP Sheep Log in to see Polyclonal

Top referenced anti-ERK2 antibody for Immunoprecipitation

Similar anti-ERK2 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine) Rat (Rattus)
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 281 Antibodies 17 Antibodies 95 Antibodies 65 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies 1 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 13 Antibodies 39 Antibodies 17 Antibodies 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies 66 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope C-Term
(60), (25), (15), (9), (8), (8), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(322), (105), (70), (36), (16), (7), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(163), (160), (16), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(8), (8), (7), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(301), (144), (89), (84), (68), (53), (50), (26), (19), (9), (5), (3), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling Images
Specificity This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2). It does not crossreact with MAP kinase 1 (ERK1).
Characteristics Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Components incl. positive Control
Immunogen Peptide conjugated to KLHEpitope: C-terminus
Clone 6G11
Isotype IgG1

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Molecular Weight 42 kDa
Gene ID 5594
UniProt P28482
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling Images back to top
Application Notes ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: 1-10 μg/mL per 10^6 pervanadate-treated A431 cells. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Images back to top
Reconstitution Restore with 1.0 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (C-Term) (ABIN126832) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody