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|Application / Reactivity||Human||Dog (Canine)||Mouse (Murine)||Rat (Rattus)|
|Simple Western (SimWes)||2 Antibodies|
|Western Blotting (WB)||281 Antibodies||17 Antibodies||95 Antibodies||65 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies||1 Antibodies|
|Luminex Assay (LMNX)||5 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies||39 Antibodies||17 Antibodies||21 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies||66 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ERK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-ERK2 AntibodyTarget Details ERK2 Application Details Handling Images
|Specificity||This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2). It does not crossreact with MAP kinase 1 (ERK1).|
|Characteristics||Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2|
|Purification||Subsequent Thiophilic Adsorption and Size Exclusion Chromatography|
|Components||incl. positive Control|
|Immunogen||Peptide conjugated to KLHEpitope: C-terminus|
Target Details ERK2Product Details anti-ERK2 Antibody Application Details Handling Images back to top
|Alternative Name||ERK2 (MAPK1 Antibody Abstract)|
|Background||Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK|
|Molecular Weight||42 kDa|
|Research Area||Stem Cells, Signaling, Inflammation|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-ERK2 Antibody Target Details ERK2 Handling Images back to top
ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: 1-10 μg/mL per 10^6 pervanadate-treated A431 cells. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Images back to top
|Reconstitution||Restore with 1.0 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling back to top