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|Application / Reactivity||Human||Rat (Rattus)||Dog (Canine)||Mouse (Murine)||Cow (Bovine)||Hamster||Guinea Pig|
|Western Blotting (WB)||498 Antibodies||105 Antibodies||34 Antibodies||112 Antibodies||36 Antibodies||24 Antibodies||27 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||120 Antibodies||28 Antibodies||29 Antibodies||28 Antibodies||24 Antibodies||23 Antibodies||22 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||108 Antibodies||26 Antibodies||27 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|8 references available|
|Supplier||Log in to see|
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN263953) Images
|Specificity||Detects a 25 kDa or 27 kDa protein corresponding to the Molecular Mass of Hsp25/27 on SDS PAGE immunoblots. Cross-reacts with Alpha B Crystallin.|
|Characteristics||Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN263953) Images back to top
|Alternative Name||HSPB1 / HSP27 (HSPB1 Antibody Abstract)|
|Background||Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN263953) Images back to top
Western blot (6): 1: 5000 (ECL). Immunoflourescence (6): 1: 200. Immunoprecipitation (9). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN263953) Images back to top
|Buffer||PBS, 0.09 % Sodium Azide, 50 % Glycerol|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-HSPB1 Antibody (ABIN263953)Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." in: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005
Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." in: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002
Jia, Ransom, Shibanuma, Liu, Welsh, Smoyer: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001
Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." in: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000
Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." in: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998
Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." in: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997
Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." in: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993
Welch: "Phorbol ester, calcium ionophore, or serum added to quiescent rat embryo fibroblast cells all result in the elevated phosphorylation of two 28,000-dalton mammalian stress proteins." in: The Journal of biological chemistry, Vol. 260, Issue 5, pp. 3058-62, 1985
ImagesProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN263953) back to top