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|Application / Reactivity||Human|
|Dot Blot (DB)||19 Antibodies|
|Enzyme Immunoassay (EIA)||15 Antibodies|
|Flow Cytometry (FACS)||35 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||94 Antibodies|
|Immunoelectron Microscopy (IEM)||2 Antibodies|
|Immunofluorescence (IF)||135 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies|
|Immunohistochemistry (IHC)||258 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||24 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||108 Antibodies|
|Immunoprecipitation (IP)||120 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|5 references available|
|Supplier||Log in to see|
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN358314) Images
|Specificity||This antibody detects HSPB1 pSer78.|
|Purification||Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser78 of human HSBP1.|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN358314) Images back to top
|Alternative Name||HSPB1 / HSP27 (HSPB1 Antibody Abstract)|
|Background||In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27|
|Molecular Weight||22783 Da|
|Gene ID||3315, 9606|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN358314) Images back to top
ELISA: 1/1,000. Dot Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN358314) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-HSPB1 Antibody (ABIN358314)Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Wano, Kita, Takahashi et al.: "Protective role of HSP27 against UVC-induced cell death in human cells." in: Experimental cell research, Vol. 298, Issue 2, pp. 584-92, 2004 (PubMed).
Evgrafov, Mersiyanova, Irobi et al.: "Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. ..." in: Nature genetics, Vol. 36, Issue 6, pp. 602-6, 2004 (PubMed).
Song, Ethier, Dziubinski et al.: "Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 143-50, 2004 (PubMed).
Chauhan, Li, Hideshima et al.: "Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance." in: Blood, Vol. 102, Issue 9, pp. 3379-86, 2003 (PubMed).
Van Why, Mann, Ardito et al.: "Hsp27 associates with actin and limits injury in energy depleted renal epithelia." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 1, pp. 98-106, 2002 (PubMed).
ImagesProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN358314) back to top