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anti-Human HSPB1 antibody for Enzyme Immunoassay

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
pSer78
66
66
52
40
25
22
16
16
13
13
12
8
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
753
274
262
87
79
78
73
54
42
39
24
22
18
8
4
4
4
2
2
1
1
1
Host
Rabbit
480
313
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA)
671
291
266
240
231
190
185
128
68
34
18
14
5
2
2
2
Supplier
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Catalog No. ABIN358314
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN359387 EIA WB Rabbit Ig Ser83 Log in to see Polyclonal 5
0.0008867729 ABIN359389 EIA IHC (p) WB Rabbit Ig Ser78 Log in to see Polyclonal 5
0.0008867729 ABIN359390 EIA IHC (p) WB Rabbit Ig Ser82 Log in to see Polyclonal 5
0.0008867729 ABIN317528 EIA IHC (p) WB Mouse IgG1 AA 1-205, N-Term Log in to see 2A5 3
0.0008867729 ABIN453119 EIA WB Rabbit Middle Region Log in to see Polyclonal 5
0.0008867729 ABIN265470 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008867729 ABIN452670 EIA IHC (p) IP WB Mouse IgG2b Log in to see 5D12-A12
0.0008867729 ABIN452669 EIA IHC (p) IP WB Mouse IgG2b Log in to see 5D12-A12
0.0008867729 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D7 2
0.0008867729 ABIN400642 EIA IF IHC (p) IP WB Mouse IgG1 Log in to see n-a
0.0008867729 ABIN1107612 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008867729 ABIN2440480 EIA IEM ICC IP IHC WB Mouse IgG1 Log in to see G3-1
0.0008867729 ABIN613191 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal
0.0008867729 ABIN613665 EIA RIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Enzyme Immunoassay

Similar anti-HSPB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
ELISA 255 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 123 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 173 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 219 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 280 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 65 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 218 Antibodies
Immunoprecipitation (IP) 165 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope pSer78
(66), (66), (52), (40), (25), (22), (16), (16), (13), (13), (12), (8), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(753), (274), (262), (87), (79), (78), (73), (54), (42), (39), (24), (22), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(480), (313), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA)
(671), (291), (266), (240), (231), (190), (185), (128), (68), (34), (18), (14), (5), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN358314) Images
Specificity This antibody detects HSPB1 pSer78.
Purification Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser78 of human HSBP1.
Isotype Ig

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN358314) Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Molecular Weight 22783 Da
Gene ID 3315, 9606
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN358314) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN358314) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-HSPB1 Antibody (ABIN358314)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Wano, Kita, Takahashi, Sugaya, Hino, Hosoya, Suzuki: "Protective role of HSP27 against UVC-induced cell death in human cells." in: Experimental cell research, Vol. 298, Issue 2, pp. 584-92, 2004

Evgrafov, Mersiyanova, Irobi, Van Den Bosch, Dierick, Leung, Schagina, Verpoorten, Van Impe, Fedotov, Dadali, Auer-Grumbach, Windpassinger, Wagner, Mitrovic, Hilton-Jones, Talbot, Martin, Vasserman et al.: "Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. ..." in: Nature genetics, Vol. 36, Issue 6, pp. 602-6, 2004

Song, Ethier, Dziubinski, Lin: "Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 143-50, 2004

Chauhan, Li, Hideshima, Podar, Mitsiades, Mitsiades, Catley, Tai, Hayashi, Shringarpure, Burger, Munshi, Ohtake, Saxena, Anderson: "Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance." in: Blood, Vol. 102, Issue 9, pp. 3379-86, 2003

Van Why, Mann, Ardito, Thulin, Ferris, Macleod, Kashgarian, Siegel: "Hsp27 associates with actin and limits injury in energy depleted renal epithelia." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 1, pp. 98-106, 2002

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN358314) back to top
Supplier Images
 image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer78) (ABIN358314) anti-Heat Shock 27kDa Protein 1 (HSPB1) (pSer78) antibody