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anti-Human HSPB1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
AA 100-150
67
66
52
40
25
22
16
16
13
12
12
8
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
753
273
261
87
79
78
73
54
42
39
24
22
18
8
4
4
4
2
2
1
1
1
Host
Rabbit
480
313
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
670
291
266
240
230
190
185
128
68
33
18
15
5
2
2
2
Supplier
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Catalog No. ABIN672441
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008846439 ABIN685303 IF (p) IHC (p) WB Rabbit IgG AA 50-100, pSer78 Log in to see Polyclonal
0.0008846439 ABIN901429 IF (p) Alexa Fluor 488 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN901431 IF (p) Alexa Fluor 555 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN901433 IF (p) Alexa Fluor 350 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN901432 IF (p) Alexa Fluor 647 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN672444 IF (p) Cy3 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN672448 IF (p) FITC Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN672445 IF (p) Cy5 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN672447 IF (p) Cy7 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN672446 IF (p) Cy5.5 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN2814545 IF (p) Alexa Fluor 594 Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008846439 ABIN877140 IF (p) Alexa Fluor 350 Rabbit IgG pSer82 Log in to see Polyclonal
0.0008846439 ABIN877142 IF (p) Alexa Fluor 555 Rabbit IgG pSer82 Log in to see Polyclonal
0.0008846439 ABIN877138 IF (p) Alexa Fluor 488 Rabbit IgG pSer82 Log in to see Polyclonal
0.0008846439 ABIN877141 IF (p) Alexa Fluor 647 Rabbit IgG pSer82 Log in to see Polyclonal
0.0008846439 ABIN743144 IF (p) Cy7 Rabbit IgG pSer15 Log in to see Polyclonal
0.0008846439 ABIN743141 IF (p) Cy3 Rabbit IgG pSer15 Log in to see Polyclonal
0.0008846439 ABIN874352 IF (p) Alexa Fluor 555 Rabbit IgG pSer15 Log in to see Polyclonal
0.0008846439 ABIN874356 IF (p) Alexa Fluor 350 Rabbit IgG pSer15 Log in to see Polyclonal
0.0008846439 ABIN874354 IF (p) Alexa Fluor 488 Rabbit IgG pSer15 Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 42 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 2 Antibodies
Flow Cytometry (FACS) 101 Antibodies 99 Antibodies
Immunocytochemistry (ICC) 124 Antibodies 136 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 146 Antibodies 156 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 91 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 126 Antibodies
Immunoprecipitation (IP) 82 Antibodies
Western Blotting (WB) 226 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 100-150
(67), (66), (52), (40), (25), (22), (16), (16), (13), (12), (12), (8), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(753), (273), (261), (87), (79), (78), (73), (54), (42), (39), (24), (22), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(480), (313), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(670), (291), (266), (240), (230), (190), (185), (128), (68), (33), (18), (15), (5), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN672441) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human HSP27
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN672441) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background

Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.

Subcellular location: Cytoplasm, Nucleus


Synonyms: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.7667, HEL-S-12, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN672441) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN672441) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-HSPB1 Antibody (ABIN672441)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Gao, Liu, Chen, Lv, Wu, Mi, Wang: "Comparative study of Hsp27, GSK3?, Wnt1 and PRDX3 in Hirschsprung's disease." in: International journal of experimental pathology, Vol. 95, Issue 3, pp. 229-37, 2014

Wang, Yang, Liu, Zhou, Wu, Qiao, Li, Wang: "Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets." in: The Journal of nutrition, Vol. 142, Issue 1, pp. 7-13, 2011

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN672441) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/H...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with (ABIN672441) Rabbit Ant...
SDS-PAGE (SDS) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27/HspB1/HSP25 Poly...
Immunofluorescence (IF) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27/HspB1/HSP25 Po...