anti-Human HSPB1 antibody for Immunohistochemistry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
C-Term
80
67
54
45
24
16
15
15
15
13
11
9
6
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
812
279
274
96
85
71
68
66
36
30
29
26
18
11
11
9
6
3
2
2
2
2
1
1
1
Host
Rabbit
498
331
2
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
33
31
22
20
13
13
13
13
11
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
701
322
270
258
223
207
165
156
59
40
17
11
11
8
6
4
2
2
1
1
1
Options
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Catalog No. ABIN2779293
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.637035 ABIN969199 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 5D7 1
9.637035 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
9.637035 ABIN933725 IHC ELISA WB Mouse IgG1 kappa AA 1-205 Log in to see 2A5 1
9.637035 ABIN1531866 IHC ELISA WB Rabbit IgG pSer15 Log in to see Polyclonal
9.637035 ABIN1532898 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.637035 ABIN1532897 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.637035 ABIN1532899 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.637035 ABIN2957626 IHC WB Rabbit pSer82 Log in to see Polyclonal
9.637035 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 8A7
9.637035 ABIN2707582 IP IHC WB Rabbit N-Term, pSer15 Log in to see Polyclonal
9.637035 ABIN2706342 IP IHC WB Rabbit Center Log in to see Polyclonal
9.637035 ABIN3181600 IHC WB Mouse IgG1 Log in to see Mix-mA
9.637035 ABIN1870247 IF IHC WB Rabbit IgG pSer15 Log in to see Polyclonal
9.637035 ABIN1870249 IF IHC WB Rabbit IgG pSer78 Log in to see Polyclonal
9.637035 ABIN1870251 IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
9.637035 ABIN3181506 IHC WB Rabbit IgG Log in to see Polyclonal
9.637035 ABIN2466960 IHC ELISA WB Mouse IgG1 AA 1-205 Log in to see 2A5
9.637035 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
9.637035 ABIN2470245 ICC IP IHC ELISA WB Mouse IgG1 lambda Log in to see
9.637035 ABIN2481411 FACS ICC IF IHC IP WB Atto 390 Mouse IgG1 kappa Log in to see 8A7

Top referenced anti-HSPB1 antibody for Immunohistochemistry

Similar anti-HSPB1 Antibodies

Application / Reactivity Human
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 261 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 156 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 202 Antibodies
Immunofluorescence (IF) 218 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 316 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope C-Term
(80), (67), (54), (45), (24), (16), (15), (15), (15), (13), (11), (9), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(812), (279), (274), (96), (85), (71), (68), (66), (36), (30), (29), (26), (18), (11), (11), (9), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(498), (331), (2)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(33), (31), (22), (20), (13), (13), (13), (13), (11), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(701), (322), (270), (258), (223), (207), (165), (156), (59), (40), (17), (11), (11), (8), (6), (4), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling ProductDetails: References for anti-HSPB1 antibody (ABIN2779293) Images
Sequence SLSPEGTLTV EAPMPKLATQ SNEITIPVTF ESRAQLGGPE AAKSDETAAK
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against HSPB1. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human HSPB1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling ProductDetails: References for anti-HSPB1 antibody (ABIN2779293) Images back to top
Antigen
Alternative Name HSPB1 (HSPB1 Antibody Abstract)
Background HSPB1, like the other heat shock proteins, is part of a complex system of molecular chaperones in epidermal keratinocytes.
Alias Symbols: CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27
Protein Interaction Partner: PCSK9, HUWE1, UBC, GATA1, MAPKAPK2, AKT1, CRYAB, MAP3K7, SUMO2, HAUS1, CEP57, AARSD1, BAG3, IRS4, HSF1, LCE3A, TGFB1I1, SUMO1, NEDD8, MDM2, ASB6, ASB10, ASB17, WWOX, ASB4, ZBTB1, RPA3, RPA2, RPA1, EZH2, ESR1, SOX2, NPM1, PLG, CS, MAPKAPK3, INS, HSPB1, SGA
Protein Size: 205
Molecular Weight 23 kDa
Gene ID 3315
NCBI Accession NM_001540, NP_001531
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling ProductDetails: References for anti-HSPB1 antibody (ABIN2779293) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 205 AA

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details ProductDetails: References for anti-HSPB1 antibody (ABIN2779293) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-HSPB1 antibody (ABIN2779293)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Sookoian, Castaño, Scian, San Martino, Pirola: "Heat Shock Protein 27 is down-regulated in Ballooned Hepatocytes of Patients with Nonalcoholic Steatohepatitis (NASH)." in: Scientific reports, Vol. 6, pp. 22528, 2016

Narjoz, Marisa, Imbeaud, Paris, Delacroix, Beaune, De Waziers: "Genomic consequences of cytochrome P450 2C9 overexpression in human hepatoma cells." in: Chemical research in toxicology, Vol. 22, Issue 5, pp. 779-87, 2009

Background publications

Lelj-Garolla, Mauk: "Self-association and chaperone activity of Hsp27 are thermally activated." in: The Journal of biological chemistry, Vol. 281, Issue 12, pp. 8169-74, 2006

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling ProductDetails: References for anti-HSPB1 antibody (ABIN2779293) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (C-Term) (ABIN2779293) Human kidney
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (C-Term) (ABIN2779293) Rabbit Anti-HSPB1 Antibody Catalog Number: ARP30177_T100 Formalin Fixed Paraffin Embe...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (C-Term) (ABIN2779293) WB Suggested Anti-HSPB1 Antibody Titration: 1.25ug/ml ELISA Titer: 1:62500 Positive C...