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anti-Monkey HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Human, Monkey
753
274
262
87
79
78
73
54
42
39
24
21
18
8
4
4
4
2
2
1
1
1
Host
Rabbit
480
313
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
670
291
266
239
231
190
184
128
68
34
18
15
5
2
2
2
Supplier
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Catalog No. ABIN222927
$ 336.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN127002 IF IP WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN1002408 IF IP WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN2439256 IF IHC WB Rabbit pSer15 Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Immunofluorescence

  • Human Polyclonal HSPB1 Primary Antibody for IF, IP - ABIN222927 (1 Publications): Cheng, Pollock, Mahimkar, Olson, Lovett: Matrix metalloproteinase 2 and basement membrane integrity: a unifying mechanism for progressive renal injury. in FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 (PubMed)

Similar anti-HSPB1 Antibodies

Application / Reactivity Monkey Human
ELISA 3 Antibodies 255 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 15 Antibodies
Flow Cytometry (FACS) 2 Antibodies 123 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 173 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies 2 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 19 Antibodies
Dot Blot (DB) 19 Antibodies
Immunochromatography (IC) 4 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Monkey
(753), (274), (262), (87), (79), (78), (73), (54), (42), (39), (24), (21), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(480), (313), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(670), (291), (266), (239), (231), (190), (184), (128), (68), (34), (18), (15), (5), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN222927) Images
Specificity The antibody detects a 27 kDa protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots, in samples from human, monkey, dog (weakly) and pig (weakly) origins.
Purification Affinity purified
Immunogen Recombinant human Hsp27
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN222927) Images back to top
Antigen
Alternative Name Hsp27 (HSPB1 Antibody Abstract)
Background Human Hsp27, mouse Hsp25 and αβ-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data sμggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
Synonyms: HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25
Gene ID 11036357
Research Area Cardiovascular, Contractility, Atherosclerosis, Heat Shock Proteins, Cytoskeleton, Microfilaments
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN222927) Images back to top
Application Notes Western blotting (1-2 μg/mL), Immunoprecipitation (5-10 μg/mL), Immunocytochemistry (10-15 μg/mL), and ELISA (1 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: USA

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN222927) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (200 μg/mL) in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-HSPB1 Antibody (ABIN222927)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Cheng, Pollock, Mahimkar, Olson, Lovett: "Matrix metalloproteinase 2 and basement membrane integrity: a unifying mechanism for progressive renal injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 11, pp. 1898-900, 2006