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anti-Mouse (Murine) HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
pSer82
63
63
50
23
22
16
16
13
12
11
8
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
613
153
139
44
37
28
25
22
18
16
9
8
5
4
3
2
1
1
1
1
Host
Rabbit
449
192
1
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
29
25
18
13
13
13
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
519
255
227
144
127
106
99
43
34
27
18
15
5
2
2
2
Supplier
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Catalog No. ABIN498746
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3452421 ABIN498848 IHC (p) Rabbit pSer78, pSer82 Log in to see Polyclonal
0.3452421 ABIN1107613 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.3452421 ABIN1107612 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.3452421 ABIN498463 IHC (p) WB Rabbit Log in to see Polyclonal
0.3452421 ABIN498937 IHC (p) Rabbit Log in to see Polyclonal
0.0008944301 ABIN672441 IF (p) IHC (p) WB Rabbit IgG AA 100-150 Log in to see Polyclonal 2
0.0008944301 ABIN197344 IHC (p) WB Rabbit Ser15 Log in to see Polyclonal 4
0.0008944301 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0008944301 ABIN672450 IHC (p) WB HRP Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008944301 ABIN672443 IHC (p) WB Biotin Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008944301 ABIN3043648 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see IAP-9
0.0008944301 ABIN1859221 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-209 Log in to see Polyclonal
0.0008944301 ABIN685320 IHC (p) WB Biotin Rabbit IgG pSer82 Log in to see Polyclonal
0.0008944301 ABIN685327 IHC (p) WB HRP Rabbit IgG pSer82 Log in to see Polyclonal
0.0008944301 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0008944301 ABIN685318 IF (p) IHC (p) WB Rabbit IgG pSer82 Log in to see Polyclonal
0.0008944301 ABIN447138 IHC (p) IHC Rabbit Ser78, Ser82 Log in to see Polyclonal
0.0008944301 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 8A7
0.0008944301 ABIN3024930 IF IHC (p) WB Mouse IgG1, kappa Log in to see SRPR27
0.0008944301 ABIN3025020 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774

Top referenced anti-HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Western Blotting (WB) 502 Antibodies 108 Antibodies 115 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 121 Antibodies 28 Antibodies 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 109 Antibodies 26 Antibodies 27 Antibodies
Immunohistochemistry (IHC) 259 Antibodies 55 Antibodies 61 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 24 Antibodies 8 Antibodies 8 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope pSer82
(63), (63), (50), (23), (22), (16), (16), (13), (12), (11), (8), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(613), (153), (139), (44), (37), (28), (25), (22), (18), (16), (9), (8), (5), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(449), (192), (1)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(29), (25), (18), (13), (13), (13), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(519), (255), (227), (144), (127), (106), (99), (43), (34), (27), (18), (15), (5), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity This antibody detects endogenous levels of p-HSP27 protein only when phosphorylated at Ser82.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. The intracellular concentration of HSP 27 increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. In addition to heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. MAP kinaseactivated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Molecular Weight approx. 27 kDa
Gene ID 3315
NCBI Accession NP_001531
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer82) (ABIN498746) Immunohistochemistry (IHC) analyzes of p-HSP27 antibody in paraffin-embedded h...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer82) (ABIN498746) Western blot (WB) analyzes of p-HSP27 antibody in extracts from HUVEC TNF cells.