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anti-Mouse (Murine) HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
pSer78, pSer82
66
65
52
40
25
22
16
16
13
13
12
8
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
752
272
261
87
79
78
73
54
42
39
24
22
18
8
4
4
4
2
2
1
1
1
Host
Rabbit
479
314
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
671
290
267
240
230
191
185
128
68
34
18
15
5
2
2
2
Supplier
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Catalog No. ABIN498848
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.25019306 ABIN498746 IHC (p) WB Rabbit pSer82 Log in to see Polyclonal
0.25019306 ABIN498463 IHC (p) WB Rabbit Log in to see Polyclonal
0.25019306 ABIN1107613 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.25019306 ABIN1107612 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.25019306 ABIN498937 IHC (p) Rabbit Log in to see Polyclonal
0.25019306 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 8A7
0.25019306 ABIN4319975 IHC IHC (p) WB Rabbit pSer82 Log in to see Polyclonal
0.0008939763 ABIN672441 IF (p) IHC (p) WB Rabbit IgG AA 100-150 Log in to see Polyclonal 2
0.0008939763 ABIN197344 IHC (p) WB Rabbit Ser15 Log in to see Polyclonal 4
0.0008939763 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0008939763 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
0.0008939763 ABIN672450 IHC (p) WB HRP Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008939763 ABIN672443 IHC (p) WB Biotin Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008939763 ABIN3043648 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see IAP-9
0.0008939763 ABIN1859221 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-209 Log in to see Polyclonal
0.0008939763 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0008939763 ABIN685327 IHC (p) WB HRP Rabbit IgG pSer82 Log in to see Polyclonal
0.0008939763 ABIN685320 IHC (p) WB Biotin Rabbit IgG pSer82 Log in to see Polyclonal
0.0008939763 ABIN685318 IF (p) IHC (p) WB Rabbit IgG pSer82 Log in to see Polyclonal
0.0008939763 ABIN4320066 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774

Top referenced anti-HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Western Blotting (WB) 635 Antibodies 226 Antibodies 230 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 163 Antibodies 82 Antibodies 83 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 215 Antibodies 123 Antibodies 125 Antibodies
Immunohistochemistry (IHC) 276 Antibodies 81 Antibodies 90 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 65 Antibodies 49 Antibodies 49 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope pSer78, pSer82
(66), (65), (52), (40), (25), (22), (16), (16), (13), (13), (12), (8), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(752), (272), (261), (87), (79), (78), (73), (54), (42), (39), (24), (22), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(479), (314), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(671), (290), (267), (240), (230), (191), (185), (128), (68), (34), (18), (15), (5), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity This antibody detects endogenous levels of HSP27 protein only when phosphorylated at Ser78/82.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress induced by insult. Heat, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. In vitro, HSP 27 becomes highly phosphorylated following exposure to stress. The discovery that HSP 27 is regulated by hormones such as estrogen has led to studies establishing a relationship between HSP 27 and breast cancer.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Molecular Weight approx. 27 kDa
Gene ID 3315
NCBI Accession NP_001531
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer78) (ABIN498848) Immunohistochemistry (IHC) analyzes of p-HSP27 antibody in paraffin-embedded h...