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|Application / Reactivity||Rat (Rattus)||Rabbit|
|Cytometry by Time of Flight (CyTOF)||8 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||101 Antibodies|
|Immunocytochemistry (ICC)||102 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||128 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||82 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||45 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||115 Antibodies||4 Antibodies|
|Immunoprecipitation (IP)||59 Antibodies||2 Antibodies|
|Western Blotting (WB)||222 Antibodies||11 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling Images
|Purpose||Mouse IgG monoclonal antibody for HSP25, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,mouse,rat,rabbit. No cross reactivity with other proteins.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Mouse IgG monoclonal antibody for HSP25, heat shock 27 kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,mouse,rat,rabbit.
Gene Name: heat shock 27 kDa protein 1
Protein Name: Heat shock protein beta-1
|Immunogen||Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
|Alternative Name||HSPB1 (HSPB1 Antibody Abstract)|
The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
Synonyms: HSP27|HSP28|CMT2F|HMN2B|28 kDa heat shock protein|Estrogen-regulated 24 kDa protein|Heat shock 27 kDa protein
|Gene ID||3315, 15507, 24471|
|UniProt||P04792, P14602, P42930|
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
WB: Concentration: 0.5-2 μg/mL, Species: Human, mouse, rabbit, rat
IHC-P: Concentration: 1-2 μg/mL, Species: Human, mouse, rabbit, rat
IHC-F: Concentration: 1-2 μg/mL, Species: Human, mouse, rabbit, rat
ICC: Concentration: 1 μg/mL, Species: Human, mouse, rabbit, rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
|Reconstitution||Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.|
|Buffer||Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.