anti-Human JNK antibody for Immunohistochemistry (Frozen Sections)

Recommended JNK Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • JNK-46
  • JNK1
  • JNK1A2
  • JNK21B1/2
  • PRKM8
  • SAPK1
  • SAPK1c
  • AI849689
  • Prkm8
  • jnk1
  • sapk1
  • T10F20.15
  • mapk8
  • zgc:112379
  • basket
  • mitogen-activated protein kinase 8
  • mitogen-activated protein kinase 8b
  • Protein JNK-1
  • bsk
  • MAPK8
  • Mapk8
  • mapk8
  • ATMPK8
  • mapk8b
  • jnk-1
Reactivity
Human, Mouse (Murine)
419
200
169
23
19
13
8
8
6
4
3
3
3
2
2
2
2
1
1
Host
Mouse
319
115
3
2
Clonality
Monoclonal
Conjugate
This JNK antibody is un-conjugated
21
19
15
13
11
11
5
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
333
210
100
76
62
61
39
37
31
22
18
8
4
3
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN126827
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126825 EIA IHC (fro) WB Biotin Mouse IgG1 N-Term Log in to see 5D10
1 ABIN126826 EIA IHC (fro) WB Mouse IgG1 N-Term Log in to see 5D10

General

Antigen Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
Reactivity Human, Mouse (Murine)
(419), (200), (169), (23), (19), (13), (8), (8), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host Mouse
(319), (115), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This JNK antibody is un-conjugated
(21), (19), (15), (13), (11), (11), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(333), (210), (100), (76), (62), (61), (39), (37), (31), (22), (18), (8), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-JNK Antibody

Target Details JNK Application Details Handling Images
Specificity This antibody specifically interacts with the Thr - Gly/Pro - pTyr motif of activated, monophosphorylated SAPK1/2 kinases. The antibody accepts the dually phosphorylated site as well (pThr - Gly/Pro - pTyr) and does not interact with the non-phosphorylated form of the protein. Mab SAPK1/2-9H8 shows no crossreaction with activated MAP Kinases 1 or 2.
Characteristics Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8,Stress-activated protein kinase JNK1
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic peptide conjugated to KLH.
Clone 9H8
Isotype IgG1

Target Details JNK

Product Details anti-JNK Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK8 / JNK1 (MAPK8 Antibody Abstract)
Background Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines.Synonyms: JNK-1, JNK-46, Mitogen-activated protein kinase 8, PRKM8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1
Gene ID 5599
UniProt P45983
Pathways MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-JNK Antibody Target Details JNK Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from sorbit-treated A431 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 cells. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-JNK Antibody Target Details JNK Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS / 0.1 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-JNK Antibody Target Details JNK Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibody (ABIN126827) anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibody