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anti-Mouse (Murine) MAP2K2 antibody for ELISA

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • AA589381
  • ATMKK2
  • CFC4
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MAPKK2
  • MEK2
  • MK1
  • MK2
  • MKK2
  • PRKMK2
  • Prkmk2
Epitope
pThr394
32
23
15
15
14
14
12
10
10
8
8
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
230
106
89
8
4
3
2
2
Host
Rabbit
189
38
9
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
10
10
9
7
7
7
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
187
79
78
39
27
26
23
15
13
11
7
4
2
1
1
Supplier
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Catalog No. ABIN1531905
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012155735 ABIN1724726 ICC FACS ELISA WB Mouse IgG1 Log in to see 7F5 1
0.0012155735 ABIN1450614 IP IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2448587 IP IHC ELISA WB Rabbit IgG Thr394 Log in to see Polyclonal
0.0012155735 ABIN1859744 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 44-313 Log in to see Polyclonal
0.0012155735 ABIN2586967 ELISA Rabbit IgG AA 35-63 Log in to see Polyclonal
0.0012155735 ABIN3182068 ELISA IHC IP WB Rabbit IgG pThr394 Log in to see Polyclonal
0.0012155735 ABIN3185526 ELISA IHC IP WB Rabbit IgG Thr38 Log in to see Polyclonal
0.0012155735 ABIN1584756 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012155735 ABIN2625058 ELISA WB Biotin Rabbit IgG AA 44-313 Log in to see Polyclonal
0.0012155735 ABIN2586961 ELISA Rabbit IgG AA 44-313 Log in to see Polyclonal
0.0012155735 ABIN2431621 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012155735 ABIN2426173 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012155735 ABIN1088845 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012155735 ABIN1088846 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012155735 ABIN1844273 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.0012155735 ABIN1885241 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0012155735 ABIN1577723 FACS IHC ELISA WB Mouse Log in to see

Top referenced anti-MAP2K2 antibody for ELISA

Similar anti-MAP2K2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 14 Antibodies 18 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Flow Cytometry (FACS) 5 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 4 Antibodies
Immunofluorescence (IF) 16 Antibodies 18 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 69 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope pThr394
(32), (23), (15), (15), (14), (14), (12), (10), (10), (8), (8), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(230), (106), (89), (8), (4), (3), (2), (2)
Host Rabbit
(189), (38), (9), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(10), (10), (9), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(187), (79), (78), (39), (27), (26), (23), (15), (13), (11), (7), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 Antibody (ABIN1531905) Images
Specificity MEK2 (Phospho-Thr394) Antibody detects endogenous levels of MEK2 only when phosphorylated at Thr394.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human MEK2 around the phosphorylation site of Thr394.
Isotype IgG

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling References for anti-MAP2K2 Antibody (ABIN1531905) Images back to top
Antigen
Alternative Name MEK2 (MAP2K2 Antibody Abstract)
Background Molecular weight of target protein: 44 kDa
Synonyms: MAP2K2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2, MEK2, MKK2, PRKMK2
Molecular Weight 44 kDa
Gene ID 5606
OMIM 115150
UniProt P36507
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling References for anti-MAP2K2 Antibody (ABIN1531905) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.465628 (NCBI Gene Symbol: MAP2K2)

Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details References for anti-MAP2K2 Antibody (ABIN1531905) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-MAP2K2 Antibody (ABIN1531905)

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling Images back to top
Background publications

Rosen, Ginty, Weber, Greenberg: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." in: Neuron, Vol. 12, Issue 6, pp. 1207-21, 1994

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 Antibody (ABIN1531905) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (pThr394) (ABIN1531905) Western blot analysis of extracts from ovary cancer, using MEK2 (Phospho-Thr394) Anti...
Immunohistochemistry (IHC) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (pThr394) (ABIN1531905) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK2...