anti-Mouse (Murine) MAPK4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAPK4 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 4 (MAPK4) Antibodies
  • ERK-4
  • ERK3
  • Erk4
  • MAPK 4
  • PRKM4
  • p63MAPK
  • A330097D03Rik
  • Erk3
  • Prkm4
  • p63Mapk
  • erk4
  • wu:fi27d11
  • zgc:55498
  • ATMPK4
  • F2N1.1
  • F2N1_1
  • MAP kinase 4
  • mitogen-activated protein kinase 4
  • MAPK4
  • Mapk4
  • mapk4
  • MPK4
Epitope
C-Term
20
16
10
8
5
5
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
80
27
8
2
2
2
2
2
1
1
1
Host
Rabbit
64
9
3
2
2
Clonality
Polyclonal
Conjugate
This MAPK4 antibody is un-conjugated
5
5
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
69
40
38
16
14
6
4
2
1
1
Supplier
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Catalog No. ABIN359633
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN658366 IHC (p) WB Rabbit Ig AA 67-94, N-Term Log in to see Polyclonal
1 ABIN5534252 IHC (p) WB Rabbit Ig Fraction AA 67-94, N-Term Log in to see Polyclonal
1 ABIN1811594 IHC (p) WB Rabbit AA 67-94 Log in to see Polyclonal
1 ABIN1841565 IHC (p) WB Rabbit AA 527-557 Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase 4 (MAPK4) Antibodies
Epitope C-Term
(20), (16), (10), (8), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(80), (27), (8), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(64), (9), (3), (2), (2)
Clonality
Polyclonal
Conjugate This MAPK4 antibody is un-conjugated
(5), (5), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(69), (40), (38), (16), (14), (6), (4), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-MAPK4 Antibody

Target Details MAPK4 Application Details Handling ProductDetails: References for anti-MAPK4 antibody (ABIN359633) Images
Specificity This antibody reacts to ERK4.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ERK4.
Isotype Ig

Target Details MAPK4

Product Details anti-MAPK4 Antibody Application Details Handling ProductDetails: References for anti-MAPK4 antibody (ABIN359633) Images back to top
Antigen
Alternative Name MAPK4 / ERK4 (MAPK4 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The CMGC group consists of 60 kinases including the cyclin-dependent kinase (CDK) and close relatives family, the MAP kinase (ERK) family, the glycogen synthase kinase 3 (GSK3) family, and the Cdc2-like kinase (CLK) family.Synonyms: Extracellular signal-regulated kinase 4, MAP kinase isoform p63, Mitogen-activated protein kinase 4, p63-MAPK
Gene ID 5596, 9606
UniProt P31152
Pathways MAPK Signaling

Application Details

Product Details anti-MAPK4 Antibody Target Details MAPK4 Handling ProductDetails: References for anti-MAPK4 antibody (ABIN359633) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK4 Antibody Target Details MAPK4 Application Details ProductDetails: References for anti-MAPK4 antibody (ABIN359633) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-MAPK4 antibody (ABIN359633)

Product Details anti-MAPK4 Antibody Target Details MAPK4 Application Details Handling Images back to top
Background publications

Robinson, Cobb: "Mitogen-activated protein kinase pathways." in: Current opinion in cell biology, Vol. 9, Issue 2, pp. 180-6, 1997

Davis: "Transcriptional regulation by MAP kinases." in: Molecular reproduction and development, Vol. 42, Issue 4, pp. 459-67, 1996

Seger, Krebs: "The MAPK signaling cascade." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 9, Issue 9, pp. 726-35, 1995

Holland, Brown, Larsen: "Biotransformation of organic sulfides--IV. Formation of chiral benzyl alkyl and phenyl alkyl sulfoxides by Helminthosporium species NRRL 4671." in: Bioorganic & medicinal chemistry, Vol. 2, Issue 7, pp. 647-52, 1995

Zhu, Zhao, Moller, Flier: "Cloning and characterization of p97MAPK, a novel human homolog of rat ERK-3." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8202-11, 1994

Images

Product Details anti-MAPK4 Antibody Target Details MAPK4 Application Details Handling ProductDetails: References for anti-MAPK4 antibody (ABIN359633) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mitogen-Activated Protein Kinase 4 (MAPK4) (C-Term) antibody (ABIN359633) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 an...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 4 (MAPK4) (C-Term) antibody (ABIN359633) Western blot analysis of anti-ERK4 Pab in mouse brain tissue lysate. ERK4 (arrow) was...