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anti-Hamster MAPK6 antibody for ELISA

Recommended MAPK6 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
  • ATMAPK6
  • ATMPK6
  • F18O19.10
  • MAP KINASE 6
  • MAP kinase 6
  • MAPK6
  • ERK3
  • ERK6
  • P38GAMMA
  • PRKM12
  • SAPK-3
  • SAPK3
  • HsT17250
  • PRKM6
  • p97MAPK
  • 2610021I23Rik
  • D130053K17Rik
  • Erk3
  • Mapk4
  • Mapk63
  • Prkm4
  • Prkm6
  • SUSERK3
  • MAP kinase 6
  • mitogen-activated protein kinase 12
  • mitogen-activated protein kinase 6
  • MPK6
  • MAPK12
  • MAPK6
  • Mapk6
Epitope
AA 683-694
26
9
6
6
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit
121
76
62
7
5
4
4
4
3
3
2
1
1
1
1
1
Host
Goat
92
25
12
2
Clonality
Polyclonal
Conjugate
This MAPK6 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
95
69
37
24
13
11
10
6
4
2
2
1
1
1
Supplier
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Catalog No. ABIN1911983
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2963392 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal

Similar anti-MAPK6 Antibodies

Application / Reactivity Human Mouse (Murine) Chicken Cow (Bovine) Dog (Canine) Hamster Horse (Equine) Pig (Porcine) Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 86 Antibodies 59 Antibodies 8 Antibodies 6 Antibodies 5 Antibodies 3 Antibodies 4 Antibodies 5 Antibodies 4 Antibodies
RNA Interference (RNAi) 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
Epitope AA 683-694
(26), (9), (6), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit
(121), (76), (62), (7), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Goat
(92), (25), (12), (2)
Clonality
Polyclonal
Conjugate This MAPK6 antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(95), (69), (37), (24), (13), (11), (10), (6), (4), (2), (2), (1), (1), (1)
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Product Details anti-MAPK6 Antibody

Target Details MAPK6 Application Details Handling Images
Brand IHC-plus™
Specificity Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4, NP_081694.1).
Purification Immunoaffinity purified
Immunogen Synthetic peptide C-HSPVGSPLKSIQ from an internal region (near C-terminus) of human MAPK6 / ERK3 (NP_002739.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Rat, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Target Details MAPK6

Product Details anti-MAPK6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK6 / ERK3 (MAPK6 Antibody Abstract)
Background Name/Gene ID: MAPK6
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK6, ERK-3, MAP kinase 6, MAPK 6, p97-MAPK, PRKM6, MAP kinase isoform p97, ERK3, HsT17250, p97MAPK
Gene ID 5597
NCBI Accession NP_002739
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation

Application Details

Product Details anti-MAPK6 Antibody Target Details MAPK6 Handling Images back to top
Application Notes Approved: ELISA (1:128000), IHC-P (10 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 μg/mL. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Restrictions For Research Use only

Handling

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. Minimize freezing and thawing.

Images

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (AA 683-694) (ABIN1911983) Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)