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anti-Human MAPK7 antibody for Enzyme Immunoassay

Recommended MAPK7 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 7 (MAPK7) Antibodies
  • A330097D03Rik
  • ATMPK7
  • Bmk1
  • BMK1
  • ERK-5
  • Erk3
  • ERK4
  • ERK5
  • Erk5
  • Erk5-T
  • F8D23.5
  • F8D23_5
  • MAP kinase 7
  • p63Mapk
  • Prkm4
  • Prkm7
  • PRKM7
Epitope
C-Term
27
14
14
13
13
13
13
13
6
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
170
98
85
1
Host
Rabbit
143
25
3
2
1
Clonality
Polyclonal
Conjugate
This MAPK7 antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
96
56
40
28
23
21
21
5
5
2
2
Supplier
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Catalog No. ABIN359634
$ 390.50
Plus shipping costs $45.00

Top referenced anti-MAPK7 antibody for Enzyme Immunoassay

Similar anti-MAPK7 Antibodies

Application / Reactivity Human
ELISA 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 39 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 97 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 7 (MAPK7) Antibodies
Epitope C-Term
(27), (14), (14), (13), (13), (13), (13), (13), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(170), (98), (85), (1)
Host Rabbit
(143), (25), (3), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK7 antibody is un-conjugated
(7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(96), (56), (40), (28), (23), (21), (21), (5), (5), (2), (2)
Pubmed 2 references available
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Product Details anti-MAPK7 Antibody

Target Details MAPK7 Application Details Handling References for anti-MAPK7 Antibody (ABIN359634) Images
Specificity This antibody reacts to ERK5.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ERK5.
Isotype Ig

Target Details MAPK7

Product Details anti-MAPK7 Antibody Application Details Handling References for anti-MAPK7 Antibody (ABIN359634) Images back to top
Antigen
Alternative Name MAPK7 / ERK5 (MAPK7 Antibody Abstract)
Background MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.Synonyms: Big MAP kinase 1, ERK4, Extracellular signal-regulated kinase 5, MAPK7, Mitogen-activated protein kinase 7
Gene ID 5598, 9606
UniProt Q13164
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK7 Antibody Target Details MAPK7 Handling References for anti-MAPK7 Antibody (ABIN359634) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK7 Antibody Target Details MAPK7 Application Details References for anti-MAPK7 Antibody (ABIN359634) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAPK7 Antibody (ABIN359634)

Product Details anti-MAPK7 Antibody Target Details MAPK7 Application Details Handling Images back to top
Background publications

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995

Lee, Ulevitch, Han: "Primary structure of BMK1: a new mammalian map kinase." in: Biochemical and biophysical research communications, Vol. 213, Issue 2, pp. 715-24, 1995

Images

Product Details anti-MAPK7 Antibody Target Details MAPK7 Application Details Handling References for anti-MAPK7 Antibody (ABIN359634) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MAPK7 antibody (Mitogen-Activated Protein Kinase 7) (C-Term) (ABIN359634) (LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the prima...
Western Blotting (WB) image for anti-MAPK7 antibody (Mitogen-Activated Protein Kinase 7) (C-Term) (ABIN359634) Western blot analysis of anti-ERK5 C-term Pab in K562 cell line lysates (35ug/lane). ...