|Application / Reactivity||Human||Chicken||Hamster||Mouse (Murine)||Rabbit||Rat (Rattus)||Xenopus laevis||Zebrafish (Danio rerio)|
|Western Blotting (WB)||142 Antibodies||1 Antibodies||2 Antibodies||92 Antibodies||2 Antibodies||67 Antibodies||1 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Staining (ICS)||4 Antibodies||4 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies||1 Antibodies||2 Antibodies||12 Antibodies||2 Antibodies||6 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies|
|Epitope||AA 310-325 Alternatives|
|Reactivity||Chicken, Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This MAPKAP Kinase 2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|10 references available|
|Supplier||Log in to see|
Product Details anti-MAPKAP Kinase 2 AntibodyTarget Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images
|Immunogen||This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to aa 310-325 of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.|
Target Details MAPKAP Kinase 2Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
|Alternative Name||MAPKAP Kinase-2 (MAPKAPK2 Antibody Abstract)|
|Background||MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-α at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.Synonyms: MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, MK2|
|Research Area||Signaling, Inflammation, Kinases/Phosphatases|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
|Application Notes||Suitable for use in Western blotting, ELISA, Immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in Immunohistochemistry and otherimmunological methods. Affinity purified anti-MAPKAP Kinase 2 is specific for MAPKAPKinase 2 and will not effectively immunoblot other isoforms of the kinase (i. e. MAPKAP K1or K3) from cell lines and/or tissues. Use a HeLa cell lysate as a positive control. MK2assays are often used and are easier and have less background than p38 kinase assays. Immunoprecipitation of MK2 using antibodies is used for this purpose. This product wasassayed by immunoblot and was found to be reactive to 20 g of crude protein extractprepared from HeLa cells. The gel was probed with Affinity Purified anti-MAPKAP Kinase2used at a dilution of 1: 2000. Use Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG(H&L) (GOAT) (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins) as a secondary|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
|Concentration||0.9 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 2-8 °C. DO NOT FREEZE! This products is photosensitive and should be protected from light.|
References for anti-MAPKAP Kinase 2 Antibody (ABIN117983)Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling Images back to top
|Product cited in:||
Concejero, Chen, Wang et al.: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." in: Transplantation, Vol. 85, Issue 3, pp. 398-406, 2008 (PubMed).
Knauf, Mutschler: "[Mechanism of action of xipamide and its classification as a 'low ceiling diuretic'. Pharmacodynamic-pharmacokinetic studies in healthy volunteers and in kidney and liver patients]" in: Arzneimittel-Forschung, Vol. 55, Issue 1, pp. 1-14, 2005 (PubMed).
Cheng, Tseng, Chang et al.: "Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2." in: Journal of cellular biochemistry, Vol. 89, Issue 3, pp. 589-602, 2003 (PubMed).
Rousseau, Morrice, Peggie et al.: "Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs." in: The EMBO journal, Vol. 21, Issue 23, pp. 6505-14, 2002 (PubMed).
Verma, Deb, Sassano et al.: "Cutting edge: activation of the p38 mitogen-activated protein kinase signaling pathway mediates cytokine-induced hemopoietic suppression in aplastic anemia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 5984-8, 2002 (PubMed).
Neininger, Kontoyiannis, Kotlyarov et al.: "MK2 targets AU-rich elements and regulates biosynthesis of tumor necrosis factor and interleukin-6 independently at different post-transcriptional levels." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3065-8, 2002 (PubMed).
Kotlyarov, Neininger, Schubert et al.: "MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis." in: Nature cell biology, Vol. 1, Issue 2, pp. 94-7, 1999 (PubMed).
Stokoe, Caudwell, Cohen et al.: "The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2." in: The Biochemical journal, Vol. 296 ( Pt 3), pp. 843-9, 1994 (PubMed).
Zu, Wu, Gilchrist et al.: "The primary structure of a human MAP kinase activated protein kinase 2." in: Biochemical and biophysical research communications, Vol. 200, Issue 2, pp. 1118-24, 1994 (PubMed).
Rouse, Cohen, Trigon et al.: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994 (PubMed).
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