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|Antigen||MAP Kinase Interacting serine/threonine Kinase 1 (MKNK1) Antibodies|
|Conjugate||This MKNK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
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Product Details anti-MKNK1 AntibodyTarget Details MKNK1 Application Details Handling References for anti-MKNK1 antibody (ABIN359308) Images
|Specificity||This antibody reacts to MKNK1.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MKNK1.|
Target Details MKNK1Product Details anti-MKNK1 Antibody Application Details Handling References for anti-MKNK1 antibody (ABIN359308) Images back to top
|Alternative Name||MKNK1 (MKNK1 Antibody Abstract)|
|Background||MKNK1 encodes a predicted 424-amino acid protein containing a sequence typical of the catalytic domain of serine/threonine kinases and a putative N-terminal nuclear localization signal sequence.C-terminal region of MKNK1 was phosphorylated and activated in vivo and in vitro by ERK1 and p38 MAP kinases, but not by JNK/SAPK. The activated ERK is essential for the activation of MKNK1 in insect cells. MKNK1 was activated following stimulation of HeLa cells with a variety of extracellular stimuli. MNK1 bind tightly to the growth factor-regulated MAP kinases, Erk1 and Erk2, and also binds strongly to the stress-activated kinase, p38.Synonyms: MAP kinase-interacting serine/threonine-protein kinase 1, MNK1|
|Gene ID||8569, 9606|
|Pathways||MAPK Signaling, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-MKNK1 Antibody Target Details MKNK1 Handling References for anti-MKNK1 antibody (ABIN359308) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MKNK1 Antibody Target Details MKNK1 Application Details References for anti-MKNK1 antibody (ABIN359308) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MKNK1 antibody (ABIN359308)Product Details anti-MKNK1 Antibody Target Details MKNK1 Application Details Handling Images back to top
Orton, Ling, Waskiewicz, Cooper, Merrick, Korneeva, Rhoads, Sonenberg, Traugh: "Phosphorylation of Mnk1 by caspase-activated Pak2/gamma-PAK inhibits phosphorylation and interaction of eIF4G with Mnk." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38649-57, 2004
ImagesProduct Details anti-MKNK1 Antibody Target Details MKNK1 Application Details Handling References for anti-MKNK1 antibody (ABIN359308) back to top