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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|BioImaging (BI)||1 Antibodies|
|ELISA||52 Antibodies||60 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||3 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||3 Antibodies|
|Immunochromatography (IC)||5 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies||12 Antibodies|
|Immunofluorescence (IF)||40 Antibodies||41 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies||35 Antibodies|
|Immunohistochemistry (IHC)||123 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||33 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||142 Antibodies|
|Antigen||Stathmin 1 (STMN1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Stathmin 1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-Stathmin 1 AntibodyTarget Details Stathmin 1 Application Details Handling Images
|Cross-Reactivity (Details)||rat,cross reactivity tested by western blot only.|
|Immunogen||A synthetic peptide corresponding to residues in C-terminus of human Stathmin was used as immunogen.|
Target Details Stathmin 1Product Details anti-Stathmin 1 Antibody Application Details Handling Images back to top
|Alternative Name||Stathmin / STMN1 (STMN1 Antibody Abstract)|
Stathmin (oncoprotein 18, op18) is a ubiquitous cytosolic phosphoprotein with variousregulatory functions in cell proliferation, differentiation signaling, and activation (1). Inparticular, stathmin is involved in the regulation of tubulin dynamics through inhibition ofmicrotubule formation and/or microtubule depolymerization. Stathmin interacts withsoulable tubulins (alpha,beta-tubulin) resulting in the formation of T2S complex whichsequesters free tubulin, impeding tubulin formation (2). Stathmin activity is regulatedthrough phosphorylation at Ser16, Ser25, Ser38 and Ser63 by various protein kinases(e.g. MAP-kinase, p34cdc2 kinase), weakening stathmin's affinity to tubulin (3). Amutation on stathmin can lead to excess build up of mitotic spindle and with possibleconsequence of unregulated cell cycles seen in cancer cells (4). Stathmin is also knownas the generic member of a protein family which includes neural proteins SCG10, SCLIPand RB3/RB3',/RB3. All members in this family exhibits tubulin bindingability. Alternate Names: anti-Lag antibody, anti-LAP 18 antibody, anti-LAP18 antibody, anti-Leukemia associatedphosphoprotein p18 antibody, anti-Metablastin antibody, anti-Oncoprotein 18 antibody,anti-OP 18 antibody, anti-OP18 antibody, anti-p18 antibody, anti-p19 antibody,anti-Phosphoprotein 19 antibody, anti-Phosphoprotein p19 antibody, anti-PP17antibody, anti-PP19 antibody, anti-PR22 antibody, anti-Pr22 protein antibody,anti-Prosolin antibody, anti-Protein Pr22 antibody, anti-SMN antibody, anti-Stathminantibody, anti-Stathmin1 antibody, anti-STMN 1 antibody, anti-STMN1 antibody.
Gene Symbol: STMN1
|Gene ID||3925, 16765, 29332|
|Pathways||MAPK Signaling, Microtubule Dynamics|
Application DetailsProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 19kDa.
Recommended dilutions: Flow Cytometry 1:100, Immunocytochemistry/Immunofluorescence 1:100-1:250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250-1:500, Immunoprecipitation 1:50, Western Blot 1:50000-200000
Protocol specific for Stathmin 1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Handling back to top