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anti-Rat (Rattus) Stathmin 1 antibody for Flow Cytometry

Recommended Stathmin 1 Antibody (supplied by: Log in to see )

Antigen
Stathmin 1 (STMN1) Antibodies
  • 19k
  • C1orf215
  • cb959
  • fj43c10
  • Lag
  • lag
  • Lap18
  • LAP18
  • lap18
  • LOC100217988
  • OP18
  • Op18
  • op18
  • P18
  • P19
  • Pig
  • Pp17
  • PP17
  • pp17
  • Pp18
  • PP19
  • Pp19
  • pp19
  • Pr22
  • PR22
  • pr22
  • prosolin
  • SMN
  • smn
  • Smn
  • stathmin
  • stmn1
  • stmn1a
  • wu:fb14e04
  • wu:fj38b07
  • wu:fj43c10
  • zgc:110159
  • zgc:136942
Epitope
C-Term
36
35
31
27
23
19
18
17
15
11
10
7
5
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
357
234
210
15
14
13
6
6
6
4
Host
Rabbit
332
19
8
Clonality
Monoclonal
Conjugate
This Stathmin 1 antibody is un-conjugated
4
4
4
3
3
3
3
3
3
3
3
3
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
279
209
115
82
64
35
20
8
7
4
3
2
2
1
Supplier
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Catalog No. ABIN258500
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Similar anti-Stathmin 1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 1 Antibodies
ELISA 60 Antibodies 69 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies 3 Antibodies
Immunochromatography (IC) 5 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 15 Antibodies 16 Antibodies
Immunofluorescence (IF) 44 Antibodies 45 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 35 Antibodies 35 Antibodies
Immunohistochemistry (IHC) 135 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 45 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 153 Antibodies

General

Antigen Stathmin 1 (STMN1) Antibodies
Epitope C-Term
(36), (35), (31), (27), (23), (19), (18), (17), (15), (11), (10), (7), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(357), (234), (210), (15), (14), (13), (6), (6), (6), (4)
Host Rabbit
(332), (19), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Stathmin 1 antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(279), (209), (115), (82), (64), (35), (20), (8), (7), (4), (3), (2), (2), (1)
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Product Details anti-Stathmin 1 Antibody

Target Details Stathmin 1 Application Details Handling Images
Cross-Reactivity (Details) rat,cross reactivity tested by western blot only.
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in C-terminus of human Stathmin was used as immunogen.
Clone EP1573Y
Isotype IgG

Target Details Stathmin 1

Product Details anti-Stathmin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Stathmin / STMN1 (STMN1 Antibody Abstract)
Background Stathmin (oncoprotein 18, op18) is a ubiquitous cytosolic phosphoprotein with variousregulatory functions in cell proliferation, differentiation signaling, and activation (1). Inparticular, stathmin is involved in the regulation of tubulin dynamics through inhibition ofmicrotubule formation and/or microtubule depolymerization. Stathmin interacts withsoulable tubulins (alpha,beta-tubulin) resulting in the formation of T2S complex whichsequesters free tubulin, impeding tubulin formation (2). Stathmin activity is regulatedthrough phosphorylation at Ser16, Ser25, Ser38 and Ser63 by various protein kinases(e.g. MAP-kinase, p34cdc2 kinase), weakening stathmin's affinity to tubulin (3). Amutation on stathmin can lead to excess build up of mitotic spindle and with possibleconsequence of unregulated cell cycles seen in cancer cells (4). Stathmin is also knownas the generic member of a protein family which includes neural proteins SCG10, SCLIPand RB3/RB3',/RB3. All members in this family exhibits tubulin bindingability. Alternate Names: anti-Lag antibody, anti-LAP 18 antibody, anti-LAP18 antibody, anti-Leukemia associatedphosphoprotein p18 antibody, anti-Metablastin antibody, anti-Oncoprotein 18 antibody,anti-OP 18 antibody, anti-OP18 antibody, anti-p18 antibody, anti-p19 antibody,anti-Phosphoprotein 19 antibody, anti-Phosphoprotein p19 antibody, anti-PP17antibody, anti-PP19 antibody, anti-PR22 antibody, anti-Pr22 protein antibody,anti-Prosolin antibody, anti-Protein Pr22 antibody, anti-SMN antibody, anti-Stathminantibody, anti-Stathmin1 antibody, anti-STMN 1 antibody, anti-STMN1 antibody.
Gene Symbol: STMN1
Gene ID 3925, 16765, 29332
Pathways MAPK Signaling, Microtubule Dynamics

Application Details

Product Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 19kDa.
Recommended dilutions: Flow Cytometry 1:100, Immunocytochemistry/Immunofluorescence 1:100-1:250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250-1:500, Immunoprecipitation 1:50, Western Blot 1:50000-200000
Protocol Protocol specific for Stathmin 1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Stathmin 1 antibody (STMN1) (C-Term) (ABIN258500) Western blot analysis on PC12 lysate using anti-Stathimin RabMAb (cat. #110-57602), d...
Immunofluorescence (IF) image for anti-Stathmin 1 antibody (STMN1) (C-Term) (ABIN258500) Immunofluorescent staining of HeLa cells using anti-Stathmin RabMAb (cat. # 110-57602).