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anti-Mouse (Murine) CD40 antibody for ELISpot (Capture)

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • 2310042E05Rik
  • AI326936
  • Bp50
  • C87049
  • C130077D06Rik
  • CDW40
  • DCTN-50
  • GMP23-48K
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • p50
  • RBP50
  • T-BAM
  • Tnfrsf5
  • TNFRSF5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine)
601
295
57
13
11
8
7
5
4
2
1
1
1
1
Host
Rat
477
206
154
24
7
2
Clonality
Monoclonal
Conjugate
This CD40 antibody is conjugated to FITC
91
90
51
24
20
17
14
12
12
11
9
8
8
8
8
8
8
8
8
8
7
6
6
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture))
558
198
197
106
88
76
70
49
48
20
19
11
8
7
6
4
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2661714
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008711731 ABIN2661713 ELISA (Capture) ELISPOT (Capture) FITC Rat IgG2a kappa Log in to see 3-23 3

Top referenced anti-CD40 antibody for ELISpot (Capture)

Similar anti-CD40 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 50 Antibodies
ELISA (Capture) 8 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 224 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Mouse (Murine)
(601), (295), (57), (13), (11), (8), (7), (5), (4), (2), (1), (1), (1), (1)
Host Rat
(477), (206), (154), (24), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is conjugated to FITC
(91), (90), (51), (24), (20), (17), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture))
(558), (198), (197), (106), (88), (76), (70), (49), (48), (20), (19), (11), (8), (7), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2661714) Images
Purification The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Clone 3-23
Isotype IgG2a kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN2661714) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154(gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN2661714) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN2661714) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD40 Antibody (ABIN2661714)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Bourgeois, Rocha, Tanchot: "A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory." in: Science (New York, N.Y.), Vol. 297, Issue 5589, pp. 2060-3, 2002

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, 1998

Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2661714) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5)  (FITC) (ABIN2661714) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody (FITC)