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anti-Mouse (Murine) CD40 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • 2310042E05Rik
  • AI326936
  • Bp50
  • C87049
  • C130077D06Rik
  • CDW40
  • DCTN-50
  • GMP23-48K
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • p50
  • RBP50
  • T-BAM
  • Tnfrsf5
  • TNFRSF5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine)
601
295
57
13
11
8
7
5
4
2
1
1
1
1
Host
Rat
477
206
154
24
7
2
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
92
90
51
24
20
17
14
12
12
11
9
8
8
8
8
8
8
8
8
8
7
6
6
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
557
198
197
105
88
76
70
49
48
20
19
11
8
8
6
3
3
3
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1176971
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008711731 ABIN2689246 IHC (f) IHC (fro) FACS IHC (zinc) Rat IgG2a, kappa Log in to see 3-23 5
0.0008711731 ABIN2689244 IHC (f) IHC (fro) FACS Rat IgG2a, kappa Log in to see 3-23 13

Top referenced anti-CD40 antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-CD40 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 50 Antibodies
ELISA (Capture) 8 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 224 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Mouse (Murine)
(601), (295), (57), (13), (11), (8), (7), (5), (4), (2), (1), (1), (1), (1)
Host Rat
(477), (206), (154), (24), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(92), (90), (51), (24), (20), (17), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(557), (198), (197), (105), (88), (76), (70), (49), (48), (20), (19), (11), (8), (8), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN1176971) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse CD40 Recombinant Protein
Clone 3-23
Isotype IgG2a, kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN1176971) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background The 3/23 clone reacts with CD40, a 40-50 kDa glycoprotein expressed on B lymphocytes and other antigen-presenting cells. CD40 has been reported to be transiently expressed on activated CD4+ and CD8+ T cells and in some mouse strains, the 3/23 mAb has been reported to react with 5-10% of T lymphocytes in adult mouse, but not neonatal, spleen. CD40 plays a key role in B-cell growth and differentiation where interactions of CD40 with its ligand, CD154, are involved in the initiation, effector, and memory stages of cell-mediated immune responses. In addition, CD40 has been reported to be involved with the triggering of NK cells and NK-T cells. Ligation of CD40 with the 3/23 antibody has been reported to induce splenic B cells to express the costimulatory molecule CD86 (B7-2). In addition, although the 3/23 antibody by itself is a weak B-cell mitogen, it has been reported to synergize markedly with mitogenic anti-IgM, anti-IgD mAb or IL-4 to promote B-cell proliferation.
Research Area Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN1176971) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN1176971) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD40 Antibody (ABIN1176971)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Bourgeois, Rocha, Tanchot: "A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory." in: Science (New York, N.Y.), Vol. 297, Issue 5589, pp. 2060-3, 2002

Turner, Rakhmilevich, Burdelya, Neal, Imboden, Sondel, Yu: "Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 89-94, 2001

French, Chan, Tutt, Glennie: "CD40 antibody evokes a cytotoxic T-cell response that eradicates lymphoma and bypasses T-cell help." in: Nature medicine, Vol. 5, Issue 5, pp. 548-53, 1999

Leifeld, Trautwein, Dumoulin, Manns, Sauerbruch, Spengler: "Enhanced expression of CD80 (B7-1), CD86 (B7-2), and CD40 and their ligands CD28 and CD154 in fulminant hepatic failure." in: The American journal of pathology, Vol. 154, Issue 6, pp. 1711-20, 1999

Tomura, Yu, Ahn, Yamashita, Yang, Ono, Hamaoka, Kawano, Taniguchi, Koezuka, Fujiwara: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, 1999

Munder, Mallo, Eichmann, Modolell: "Murine macrophages secrete interferon gamma upon combined stimulation with interleukin (IL)-12 and IL-18: A novel pathway of autocrine macrophage activation." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2103-8, 1998

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, 1998

Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994

Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." in: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, 1994

Klaus, Holman, Hasbold: "Properties of mouse CD40: the role of homotypic adhesion in the activation of B cells via CD40." in: European journal of immunology, Vol. 24, Issue 11, pp. 2714-9, 1994

Parry, Hasbold, Holman, Klaus: "Hypercross-linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL-4 and anti-CD40." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2821-9, 1994

Parry, Holman, Hasbold, Klaus: "Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes." in: European journal of immunology, Vol. 24, Issue 4, pp. 974-9, 1994

Noelle, Ledbetter, Aruffo: "CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation." in: Immunology today, Vol. 13, Issue 11, pp. 431-3, 1993