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Human CD40 ELISA Kit

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Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) ELISA Kits
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Epitope
AA 21-193
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
26
21
3
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN921089
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666156 ABIN1888487 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 50-40000 pg/mL 50 pg/mL Log in to see
0.32666156 ABIN2708158 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Urine 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32666156 ABIN2113436 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Saliva, Urine, Milk Log in to see
0.00121214 ABIN832450 Sandwich ELISA Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.00121214 ABIN3032902 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 54.9-40000 pg/mL 54.9 pg/mL Log in to see
0.00121214 ABIN624956 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.00121214 ABIN1380475 Cell ELISA Log in to see
0.00121214 ABIN1112575 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.00121214 ABIN1380999 Cell ELISA Log in to see
0.00121214 ABIN2683737 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Milk, Serum, Saliva, Urine 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.00121214 ABIN2683738 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Amniotic Fluid, Cell Culture Supernatant, Serum 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.00121214 ABIN2065525 Cell ELISA Log in to see
0.00121214 ABIN2042400 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.00121214 ABIN2683739 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.00121214 ABIN2065523 Cell ELISA Log in to see
0.00121214 ABIN1033841 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (heparin), Amniotic Fluid Log in to see
0.00121214 ABIN1846880 Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.00121214 ABIN2683740 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
0.00121214 ABIN2869896 Log in to see
0.00121214 ABIN1573785 31.2-2000 pg/mL 31.2 pg/mL Log in to see

Top referenced CD40 ELISA Kit for ELISA

Similar CD40 ELISA Kits

Application / Reactivity Human
ELISA 27 ELISA Kits

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) ELISA Kits
Epitope AA 21-193
Reactivity Human
Kits with alternative reactivity to:
(26), (21), (3), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details CD40 ELISA Kit

Target details Application Details Handling References for CD40 Kit (ABIN921089) Images
Purpose For quantitative detection of human CD40 in cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk
Detection Method Colorimetric
Specificity Natural and recombinant human CD40
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- human CD40 antibod - 1
  • Lyophilized recombinant human CD40 standar - 10ng/tubex2
  • Biotinylated anti- human CD40 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: E21-R193

Target details

Product Details CD40 ELISA Kit Application Details Handling References for CD40 Kit (ABIN921089) Images back to top
Antigen
Alternative Name CD40 (CD40 ELISA Kit Abstract)
Background CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Gene ID 958
UniProt P25942
Research Area Adaptive Immunity, Apoptosis/Necrosis, CD Antigens, Cancer, Hematopoietic Progenitors, Inflammation, Stem Cells, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details CD40 ELISA Kit Target details Handling References for CD40 Kit (ABIN921089) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human CD40 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD40 has been precoated onto 96-well plates. Standards(NSO, E21-R193) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD40 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CD40 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD40 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 93, Standard deviation: 4.8, CV(%): 5.2
  • Sample 2: n=16, Mean(pg/ml): 325, Standard deviation: 19.2, CV(%): 5.9
  • Sample 3: n=16, Mean(pg/ml): 538, Standard deviation: 28.5, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 101, Standard deviation: 6.9, CV(%): 6.8
  • Sample 2: n=24, Mean(pg/ml): 357, Standard deviation: 26.4, CV(%): 7.4
  • Sample 3: n=24, Mean(pg/ml): 529, Standard deviation: 32.8, CV(%): 6.2
Restrictions For Research Use only

Handling

Product Details CD40 ELISA Kit Target details Application Details References for CD40 Kit (ABIN921089) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CD40 Kit (ABIN921089)

Product Details CD40 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Lafage-Pochitaloff, Herman, Birg, Galizzi, Simonetti, Mannoni, Banchereau: "Localization of the human CD40 gene to chromosome 20, bands q12-q13.2." in: Leukemia, Vol. 8, Issue 7, pp. 1172-5, 1994

Images

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Supplier Images
ELISA image for CD40 ELISA Kit (CD40 Molecule, TNF Receptor Superfamily Member 5) (ABIN921089) Human CD40/TNFRSF5 PicoKine ELISA Kit standard curve