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anti-Human EGF antibody for Functional Studies

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Human
313
124
76
15
13
7
4
3
2
1
Host
Rabbit
281
140
29
11
7
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
48
24
22
10
8
8
6
6
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
305
274
91
68
46
41
26
23
19
17
17
14
11
7
6
5
4
2
2
2
2
1
Supplier
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Catalog No. ABIN115993
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008771965 ABIN115994 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
0.0008771965 ABIN205919 Func IHC (p) ELISA WB Rabbit IgG Extracellular Domain Log in to see Polyclonal
0.0008771965 ABIN2473397 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0008771965 ABIN640563 Func IHC (p) ELISA WB Rabbit Log in to see

Top referenced anti-EGF antibody for Functional Studies

Similar anti-EGF Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 194 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 72 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 10 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(313), (124), (76), (15), (13), (7), (4), (3), (2), (1)
Host Rabbit
(281), (140), (29), (11), (7), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(48), (24), (22), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(305), (274), (91), (68), (46), (41), (26), (23), (19), (17), (17), (14), (11), (7), (6), (5), (4), (2), (2), (2), (2), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity Human Epidermal Growth Factor (hEGF)
Purification Affinity Chromatography using an immobilized Human EGF matrix
Immunogen Highly pure (>98%) E.coli derived recombinant Human EGF (hEGF).

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Gene ID 1950
NCBI Accession NP_001954
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes ELISA: To detect hEGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhEGF (2.0 ng/mL), a concentration of 0.25-0.35 μg/mL of this antibody is required. Immunohistochemistry: A starting dilution of 1 μg/mL is recommended for frozen sections. For paraffin embedded sections use microwave pretreatment, EDTA, pH 9 and a dilution of1-5 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Centrifuge vial prior to opening!
Storage 4 °C