anti-Rat (Rattus) EGF antibody for ELISA

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Epitope
AA 974-1026
26
16
15
12
12
11
10
10
9
8
8
7
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
338
164
87
19
14
7
4
2
2
1
1
1
Host
Rabbit
309
151
29
24
6
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
47
27
22
12
10
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
352
288
128
91
54
33
30
28
26
25
19
14
10
7
6
5
4
3
2
2
2
1
1
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Catalog No. ABIN5518908
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1453236 IHC ELISA WB Rabbit IgG AA 660-709 Log in to see Polyclonal
1 ABIN465662 IHC Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2692016 IHC (p) ELISA Rabbit IgG AA 974-1026 Log in to see Polyclonal
1 ABIN346005 ICC ELISA WB Sheep Internal Region Log in to see Polyclonal
1 ABIN465685 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN2885862 IHC ELISA WB Rabbit IgG AA 660-709 Log in to see Polyclonal
1 ABIN2599654 ELISA WB Rabbit IgG AA 974-1026 Log in to see Polyclonal
1 ABIN293787 ELISA WB Sheep IgG Log in to see Polyclonal
1 ABIN1819319 Func ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2428071 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2692013 ELISA Rabbit IgG AA 974-1026 Log in to see Polyclonal
1 ABIN5506794 ELISA Mouse IgG1 AA 974-1026 Log in to see 02
1 ABIN2421523 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2473399 Func ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN3201446 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
1 ABIN1002257 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2260977 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2416059 IF IP ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN5506795 ELISA Rabbit IgG AA 974-1026 Log in to see 005

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 974-1026
(26), (16), (15), (12), (12), (11), (10), (10), (9), (8), (8), (7), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(338), (164), (87), (19), (14), (7), (4), (2), (2), (1), (1), (1)
Host Rabbit
(309), (151), (29), (24), (6), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(47), (27), (22), (12), (10), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(352), (288), (128), (91), (54), (33), (30), (28), (26), (25), (19), (14), (10), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(Egf) detection. Tested with WB, IHC-P, ELISA in Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(Egf) detection. Tested with WB, IHC-P, ELISA in Rat.
Gene Name: epidermal growth factor
Protein Name: Pro-epidermal growth factor
Purification Immunogen affinity purified.
Immunogen E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.
Isotype IgG

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Egf (EGF Antibody Abstract)
Background EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms: Pro-epidermal growth factor, EGF, Epidermal growth factor, Egf
Gene ID 25313
UniProt P07522
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.