You are viewing an incomplete version of our website. Please click to reload the website as full version.

Rat (Rattus) EGF ELISA Kit

Recommended EGF Product (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) ELISA Kits
  • AI790464
  • CEGF
  • HOMG4
  • URG
Epitope
AA 974-1026
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
40
28
21
9
6
4
4
4
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
7.8-500 pg/mL
Minimum Detection Limit
7.8 pg/mL
Application
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1672859
$ 399.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN416392 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 4
0.32693866 ABIN924832 Sandwich ELISA Cell Lysate, Serum, Plasma 8-1000 pg/mL 8 pg/mL Log in to see 1
0.32693866 ABIN2472105 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN1114787 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN1888537 Sandwich ELISA Serum, Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Urine, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.32693866 ABIN2708638 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate, Urine 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012117105 ABIN365552 Sandwich ELISA Serum, Plasma, Tissue Homogenate 3.12-200 pg/mL 3.12 pg/mL Log in to see 1
0.0012117105 ABIN3032864 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.82-200 pg/mL 0.82 pg/mL Log in to see
0.0012117105 ABIN578790 Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN1979553 Serum, Plasma, Cell Culture Supernatant Log in to see 1
0.0012117105 ABIN626974 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN1569543 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012117105 ABIN510091 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN2042445 Sandwich ELISA 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012117105 ABIN2113818 Cell Lysate, Serum, Plasma 8-1000 pg/mL 8 pg/mL Log in to see
0.0012117105 ABIN2684094 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum, Tissue Homogenate, Urine 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012117105 ABIN2533055 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012117105 ABIN2108596 Sandwich ELISA Log in to see
0.0012117105 ABIN2947286 15.625-1000 pg/mL 15.625 pg/mL Log in to see

Top referenced EGF ELISA Kit for ELISA

Similar EGF ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 20 ELISA Kits

General

Antigen Epidermal Growth Factor (EGF) ELISA Kits
Epitope AA 974-1026
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(40), (28), (21), (9), (6), (4), (4), (4), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 7.8-500 pg/mL
Minimum Detection Limit 7.8 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details EGF ELISA Kit

Target details Application Details Handling References for EGF Kit (ABIN1672859) Images
Purpose For quantitative detection of rat EGF in cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates and urine.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine
Detection Method Colorimetric
Specificity Natural and recombinant rat EGF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- rat EGF antibod - 1
  • Lyophilized recombinant rat EGF standar - 10ng/tubex2
  • Biotinylated anti- rat EGF antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: N974-R1026

Target details

Product Details EGF ELISA Kit Application Details Handling References for EGF Kit (ABIN1672859) Images back to top
Antigen
Alternative Name EGF (EGF ELISA Kit Abstract)
Background Epidermal growth factor(EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
Gene ID 25313
UniProt P07522
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details EGF ELISA Kit Target details Handling References for EGF Kit (ABIN1672859) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat EGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGF has been precoated onto 96-well plates. Standards(E.coli, N974-R1026) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat EGF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat EGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat EGF standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 32.1, Standard deviation: 1.35, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 104, Standard deviation: 5.62, CV(%): 5.4
  • Sample 3: n=16, Mean(pg/ml): 256, Standard deviation: 11.52, CV(%): 4.5,
  • Sample 1: n=24, Mean(pg/ml): 35.2, Standard deviation: 2.36, CV(%): 6.7
  • Sample 2: n=24, Mean(pg/ml): 112, Standard deviation: 8.4, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 287, Standard deviation: 18.08, CV(%): 6.3
Restrictions For Research Use only

Handling

Product Details EGF ELISA Kit Target details Application Details References for EGF Kit (ABIN1672859) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for EGF Kit (ABIN1672859)

Product Details EGF ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Russo, Linsalata, Clemente, DAttoma, Orlando, Campanella, Giotta, Riezzo: "The effects of fluorouracil, epirubicin, and cyclophosphamide (FEC60) on the intestinal barrier function and gut peptides in breast cancer patients: an observational study." in: BMC cancer, Vol. 13, pp. 56, 2013

Ge, Yu, Petitte, Zhang: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." in: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, 2009

Background publications

Herbst: "Review of epidermal growth factor receptor biology." in: International journal of radiation oncology, biology, physics, Vol. 59, Issue 2 Suppl, pp. 21-6, 2004

Images

Product Details EGF ELISA Kit Target details Application Details Handling References for EGF Kit (ABIN1672859) back to top
Supplier Images
ELISA image for EGF ELISA Kit (Epidermal Growth Factor) (ABIN1672859) Rat EGF PicoKine ELISA Kit standard curve