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anti-Human EGFR antibody for Flow Cytometry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
AA 1004-1033, C-Term
146
137
57
57
53
51
49
48
46
45
44
44
35
33
32
30
30
25
24
23
23
23
23
22
22
19
18
17
16
14
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
8
8
8
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2147
741
607
21
14
14
12
10
7
7
4
2
1
1
1
Host
Rabbit
1373
687
60
44
4
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
93
73
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1311
565
504
441
414
243
232
180
86
74
67
55
25
11
11
9
8
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN651628
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30379575 ABIN1687352 FACS IHC (p) Rabbit IgG C-Term Log in to see SP9 2
0.30379575 ABIN1724889 FACS ELISA WB Mouse IgG2b AA 693-893 Log in to see 5E10D3 2
0.30379575 ABIN1724890 FACS IHC ELISA WB Mouse IgG1 AA 693-893 Log in to see 7A6F12 2
0.30379575 ABIN1724825 FACS IHC ELISA WB Mouse IgG1 AA 693-893, Mutant Log in to see 5G9B5 2
0.30379575 ABIN1724824 FACS IHC ELISA WB Mouse IgG1 AA 693-893, Mutant Log in to see 5G9B5 2
0.30379575 ABIN1381744 cELISA FACS Mouse IgG1 Log in to see DP-4A1 1
0.30379575 ABIN2161231 FACS PE Rat IgG2a Extracellular Domain Log in to see
0.30379575 ABIN2659343 FACS PE-Cy7 Mouse IgG1 kappa Log in to see AY13 4
0.30379575 ABIN2657498 FACS ELISA WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see AY13 4
0.30379575 ABIN2660176 FACS IF IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see AY13 4
0.30379575 ABIN2662712 FACS PE Mouse IgG1 kappa Log in to see AY13 5
0.30379575 ABIN153598 FACS FITC Rat IgG2a Extracellular Domain Log in to see ICR10
0.30379575 ABIN2167417 FACS FITC Rat IgG2a Extracellular Domain Log in to see
0.30379575 ABIN2664938 FACS Mouse IgG1 kappa Log in to see AY13 4
0.30379575 ABIN2473409 FACS PE Rat IgG2a Extracellular Domain Log in to see ICR10 4
0.30379575 ABIN1995318 FACS PE Mouse IgG1 AA 1-645 Log in to see 8
0.30379575 ABIN1995316 FACS FITC Mouse IgG1 AA 1-645 Log in to see 8
0.30379575 ABIN153570 FACS IHC (fro) IHC (p) IP IHC Rat IgG2a Extracellular Domain Log in to see ICR10
0.30379575 ABIN2166934 IHC (af) FACS IP Rat IgG2a Extracellular Domain Log in to see
0.30379575 ABIN2160826 FACS IHC WB Mouse IgG1 kappa Log in to see 51CT78-40-5

Top referenced anti-EGFR antibody for Flow Cytometry

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Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 86 Antibodies
ELISA 557 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 510 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 25 Antibodies
Immunocytochemistry (ICC) 74 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 189 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 1004-1033, C-Term
(146), (137), (57), (57), (53), (51), (49), (48), (46), (45), (44), (44), (35), (33), (32), (30), (30), (25), (24), (23), (23), (23), (23), (22), (22), (19), (18), (17), (16), (14), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2147), (741), (607), (21), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Rabbit
(1373), (687), (60), (44), (4), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(93), (73), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1311), (565), (504), (441), (414), (243), (232), (180), (86), (74), (67), (55), (25), (11), (11), (9), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN651628) Images
Specificity This EGFR-S1026 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1010-1029 amino acids from the C-terminal region of human IPO8.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This EGFR-S1026 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1004-1033 AA from the C-terminal region of human EGFR-S1026.
Clone RB26905
Isotype Ig

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN651628) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Synonyms: EGFR,ERBB1
Molecular Weight 134277 DA
Gene ID 1956
NCBI Accession NP_005219, NP_958439, NP_958440, NP_958441
UniProt P00533
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Cancer, Chromatin, Signaling
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN651628) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN651628) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-EGFR Antibody (ABIN651628)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN651628) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1004-1033) (ABIN651628) EGFR-S1026 (ABIN651628) western blot analysis in K562 cell line lysates (35 µg/lane)....
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1004-1033) (ABIN651628) EGFR-S1026 Antibody (C-term) (ABIN651628) immunohistochemistry analysis in formalin f...
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1004-1033) (ABIN651628) EGFR-S1026 Antibody (C-term) (ABIN651628) flow cytometric analysis of K562 cells (rig...