anti-Human EGFR antibody for Flow Cytometry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • LOC5564544
Epitope
AA 693-893, Mutant
147
135
63
63
59
57
56
54
53
52
48
48
43
39
36
33
30
30
29
27
26
25
25
24
22
19
18
18
17
16
16
16
16
15
15
15
15
15
14
14
13
11
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2404
850
684
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1444
858
81
44
9
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
102
80
71
63
56
54
52
32
32
28
28
28
28
28
28
26
26
25
24
24
21
21
21
21
21
20
19
18
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1432
677
614
556
501
272
259
229
124
84
66
56
40
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN1724825
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.112912 ABIN651628 FACS IHC (p) WB Rabbit Ig AA 1004-1033, C-Term Log in to see Polyclonal 1
14.112912 ABIN153570 FACS IHC (fro) IHC (p) IP IHC Rat IgG2a Log in to see ICR10 1
14.112912 ABIN1687352 FACS IHC (p) Rabbit IgG C-Term Log in to see SP9 2
14.112912 ABIN4896905 FACS Fluorescein Rat IgG2a AA 25-645 Log in to see 423103 1
14.112912 ABIN4896907 FACS PE Rat IgG2a AA 25-645 Log in to see 423103 1
14.112912 ABIN4899924 ELISA (Detection) FACS WB Biotin Goat IgG AA 25-645 Log in to see Polyclonal 4
14.112912 ABIN4900626 CyTOF FACS WB Mouse IgG2a pTyr1068 Log in to see 338324 2
14.112912 ABIN1724889 FACS ELISA WB Mouse IgG2b AA 693-893 Log in to see 5E10D3 2
14.112912 ABIN1724890 FACS IHC ELISA WB Mouse IgG1 AA 693-893 Log in to see 7A6F12 2
14.112912 ABIN4900636 CyTOF FACS ICC IHC WB Rabbit IgG pTyr845 Log in to see Polyclonal 2
14.112912 ABIN4899925 CyTOF ELISA (Capture) FACS ICC IHC IP WB Goat IgG AA 25-645 Log in to see Polyclonal 6
14.112912 ABIN4900628 CyTOF FACS ICC IHC SimWes WB Rabbit IgG pTyr1173 Log in to see Polyclonal 4
14.112912 ABIN153598 FACS FITC Rat IgG2a Log in to see ICR10 1
14.112912 ABIN1724824 FACS IHC ELISA WB Mouse IgG1 AA 693-893, Mutant Log in to see 5G9B5 2
14.112912 ABIN2473409 FACS PE Rat IgG2a Extracellular Domain Log in to see ICR10 4
14.112912 ABIN4896908 FACS Alexa Fluor 647 Rat IgG2a AA 25-645 Log in to see 423103
14.112912 ABIN1381744 cELISA FACS Mouse IgG1 Log in to see DP-4A1 1
14.112912 ABIN1995316 FACS FITC Mouse IgG1 AA 1-645 Log in to see 08
14.112912 ABIN4307221 FACS IF IHC (p) Mouse IgG2a kappa Log in to see GFR450
14.112912 ABIN4898093 FACS PE Mouse IgG2a pTyr1068 Log in to see 338324

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 693-893, Mutant
(147), (135), (63), (63), (59), (57), (56), (54), (53), (52), (48), (48), (43), (39), (36), (33), (30), (30), (29), (27), (26), (25), (25), (24), (22), (19), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2404), (850), (684), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1444), (858), (81), (44), (9), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(102), (80), (71), (63), (56), (54), (52), (32), (32), (28), (28), (28), (28), (28), (28), (26), (26), (25), (24), (24), (21), (21), (21), (21), (21), (20), (19), (18), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1432), (677), (614), (556), (501), (272), (259), (229), (124), (84), (66), (56), (40), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN1724825) Images
Characteristics Mutant
Purification Purified
Immunogen Purified recombinant fragment of human EGFR mutant (AA 693-893) expressed in E. Coli.
Clone 5G9B5
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN1724825) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. ,
Molecular Weight 175 kDa
Gene ID 1956
HGNC 1956
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN1724825) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN1724825) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-EGFR antibody (ABIN1724825)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Derer, Bauer, Lohse, Scheel, Berger, Kellner, Peipp, Valerius: "Impact of epidermal growth factor receptor (EGFR) cell surface expression levels on effector mechanisms of EGFR antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 11, pp. 5230-9, 2012

Minder, Bayha, Becker-Pauly, Sterchi: "Meprin? transactivates the epidermal growth factor receptor (EGFR) via ligand shedding, thereby enhancing colorectal cancer cell proliferation and migration." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35201-11, 2012

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN1724825) back to top
Supplier Images
ELISA image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (ABIN1724825) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody
Flow Cytometry (FACS) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (ABIN1724825) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (Image 2)
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (ABIN1724825) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (Image 3)
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (ABIN1724825) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (Image 4)
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (ABIN1724825) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody (Image 5)