anti-Human EGFR antibody for Immunocytochemistry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 25-645
148
122
62
59
57
53
53
51
48
48
48
43
38
36
33
30
29
27
26
25
25
25
23
22
21
19
19
18
18
17
16
16
16
15
15
15
15
15
15
15
14
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2334
850
699
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Goat
1435
798
67
44
8
3
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
75
66
60
51
49
27
27
26
25
25
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
1362
632
572
529
441
272
245
229
110
84
67
56
35
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN4899925
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.054808 ABIN4900628 FACS ICC IHC SimWes WB Rabbit IgG pTyr1173 Log in to see Polyclonal 4
26.054808 ABIN4900636 FACS ICC IHC WB Rabbit IgG pTyr845 Log in to see Polyclonal 2
1 ABIN152027 ICC IF IHC IHC (p) IP WB Rabbit AA 1100-1150 Log in to see Polyclonal 4
1 ABIN152028 ICC IF IHC IHC (p) IP PLA WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN265742 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 29-1 2
1 ABIN4307205 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN343626 ICC WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN2854848 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN780370 FACS ICC IHC (f) IP Mouse IgG2b Extracellular Domain Log in to see EGFR1 3
1 ABIN1027691 IHC (f) ICC FACS IP PE Mouse IgG2b Extracellular Domain Log in to see EGFR1 4
1 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN457474 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
1 ABIN269687 ICC FACS IF IP WB Mouse IgG2b Log in to see 6F1
1 ABIN1491514 ICC IF ELISA WB Mouse IgG2b, kappa Log in to see AT2H8
1 ABIN258577 FACS ICC IF IHC IHC (p) IP Mouse IgG2a Log in to see 528
1 ABIN2736680 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN5542766 ICC IP WB Mouse IgG1 Log in to see 5E10-G10-E11
1 ABIN5542767 ICC IP WB Mouse IgG1 Log in to see 6H11-2D11-G3
1 ABIN235070 ICC IP ELISA WB Mouse IgG1 AA 1165-1186 Log in to see 13G8
1 ABIN1858694 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 713-984 Log in to see

Top referenced anti-EGFR antibody for Immunocytochemistry

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Cell Culture (CC) 1 Antibodies
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Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 35 Antibodies
Dot Blot (DB) 84 Antibodies
ELISA 559 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
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Flow Cytometry (FACS) 630 Antibodies
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Immunocytochemistry (ICC) 109 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 25-645
(148), (122), (62), (59), (57), (53), (53), (51), (48), (48), (48), (43), (38), (36), (33), (30), (29), (27), (26), (25), (25), (25), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (14), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2334), (850), (699), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Goat
(1435), (798), (67), (44), (8), (3), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(97), (75), (66), (60), (51), (49), (27), (27), (26), (25), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(1362), (632), (572), (529), (441), (272), (245), (229), (110), (84), (67), (56), (35), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN4899925) Images
Specificity Detects human EGFR in ELISAs and Western blots. In sandwich ELISAs, approximately 3 % cross-reactivity with recombinant mouse EGFR is observed and less than 0.1 % cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EGFR, Leu25-Ser645, Accession # CAA25240
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN4899925) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Alternative Names: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene ID 1956
Research Area Receptors, Growth Factors, Hormones, Cancer, Tyrosine Kinases
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling ProductDetails: References for anti-EGFR antibody (ABIN4899925) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 1-15 μg/mL
Immunoprecipitation 1 μg/mL
Immunocytochemistry 1-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details ProductDetails: References for anti-EGFR antibody (ABIN4899925) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-EGFR antibody (ABIN4899925)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Burke, Zsengellér, Khankin, Lo, Rajakumar, DuPont, McCurley, Moss, Zhang, Clark, Wang, Seely, Kang, Stillman, Jaffe, Karumanchi: "Soluble fms-like tyrosine kinase 1 promotes angiotensin II sensitivity in preeclampsia." in: The Journal of clinical investigation, Vol. 126, Issue 7, pp. 2561-74, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Nanjo, Yamada, Nishihara, Takeuchi, Sano, Nakagawa, Ishikawa, Zhao, Ebi, Yasumoto, Matsumoto, Yano: "Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors." in: PLoS ONE, Vol. 8, Issue 12, pp. e84700, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sarup, Jin, Turin, Bai, Beryt, Brdlik, Higaki, Jorgensen, Lau, Lindley, Liu, Ni, Rozzelle, Kumari, Watson, Zhang, Shepard: "Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts." in: Molecular cancer therapeutics, Vol. 7, Issue 10, pp. 3223-36, 2008 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Gonzalez, Seurynck-Servoss, Crowley, Brown, Omenn, Hayes, Zangar: "Development and validation of sandwich ELISA microarrays with minimal assay interference." in: Journal of proteome research, Vol. 7, Issue 6, pp. 2406-14, 2008 (Sample species: Human).

Kunii, Davis, Gorenstein, Hatch, Yashiro, Di Bacco, Elbi, Lutterbach: "FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival." in: Cancer research, Vol. 68, Issue 7, pp. 2340-8, 2008 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Murphy, Ghosh, Fulford, Khwaja, Halka, Carter, Turner, Walker: "Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration." in: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, Vol. 31, Issue 5, pp. 516-22, 2006 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN4899925) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithe...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) EGF R/ErbB1 in Human Prostate Cancer Tissue. EGF R/ErbB1 was detected in immersion fi...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) Detection of Human EGF R/ErbB1 by Western Blot. Western blot shows lysates of HeLa hu...
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithe...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) Detection of Human EGF R/ErbB1 by Western Blot. Western blot shows lysates of HeLa hu...
Immunocytochemistry (ICC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) EGF R/ErbB1 in A431 Human Cell Line. EGF R/ErbB1 was detected in immersion fixed A431...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) EGF R/ErbB1 in Human Skin. EGF R/ErbB1 was detected in immersion fixed frozen section...