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anti-Human EGFR antibody for Immunocytochemistry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pTyr845
148
122
61
58
54
53
52
50
48
48
48
43
36
35
33
30
29
26
25
25
24
23
23
22
21
19
19
18
18
17
16
16
16
15
15
15
15
15
15
14
13
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2256
847
699
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Rabbit
1386
768
67
43
9
2
1
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
74
66
59
51
49
27
27
26
25
24
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
1310
604
559
486
436
272
222
222
108
84
66
56
35
24
19
14
11
8
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN4900636
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.705849 ABIN4900628 FACS ICC IHC SimWes WB Rabbit IgG pTyr1173 Log in to see Polyclonal 4
1 ABIN152027 ICC IF IHC IHC (p) IP WB Rabbit AA 1100-1150 Log in to see Polyclonal 4
1 ABIN265742 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 29-1 2
1 ABIN152028 ICC IF IHC IHC (p) IP PLA WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN4899925 ELISA (Capture) FACS ICC IHC IP WB Goat IgG AA 25-645 Log in to see Polyclonal 6
1 ABIN343626 ICC WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN4307205 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN780370 FACS ICC IHC (f) IP Mouse IgG2b Extracellular Domain Log in to see EGFR1 3
1 ABIN2854848 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1027691 IHC (f) ICC FACS IP PE Mouse IgG2b Extracellular Domain Log in to see EGFR1 4
1 ABIN269687 ICC FACS IF IP WB Mouse IgG2b Log in to see 6F1
1 ABIN457474 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
1 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1491514 ICC IF ELISA WB Mouse IgG2b, kappa Log in to see AT2H8
1 ABIN235070 ICC IP ELISA WB Mouse IgG1 AA 1165-1186 Log in to see 13G8
1 ABIN258577 FACS ICC IF IHC IHC (p) IP Mouse IgG2a Log in to see 528
1 ABIN2736680 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN5542766 ICC IP WB Mouse IgG1 Log in to see 5E10-G10-E11
1 ABIN5542767 ICC IP WB Mouse IgG1 Log in to see 6H11-2D11-G3
1 ABIN364308 ICC IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal

Top referenced anti-EGFR antibody for Immunocytochemistry

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Application / Reactivity Human
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Blocking Reagent (BR) 7 Antibodies
Cell Culture (CC) 1 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 35 Antibodies
Dot Blot (DB) 84 Antibodies
ELISA 546 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
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Flow Cytometry (FACS) 604 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 19 Antibodies
Immunocytochemistry (ICC) 107 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr845
(148), (122), (61), (58), (54), (53), (52), (50), (48), (48), (48), (43), (36), (35), (33), (30), (29), (26), (25), (25), (24), (23), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2256), (847), (699), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1386), (768), (67), (43), (9), (2), (1), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(97), (74), (66), (59), (51), (49), (27), (27), (26), (25), (24), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(1310), (604), (559), (486), (436), (272), (222), (222), (108), (84), (66), (56), (35), (24), (19), (14), (11), (8), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN4900636) Images
Specificity Detects human EGF R/ErbB1 when phosphorylated at Y845.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Immunogen Phosphopeptide containing human EGF R/ErbB1 Y845 site
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN4900636) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Alternative Names: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGFR, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene ID 1956
Research Area Receptors, Growth Factors, Hormones, Cancer, Tyrosine Kinases
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN4900636) Images back to top
Application Notes Western Blot 0.5 μg/mL
Immunohistochemistry 5-15 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN4900636) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EGFR Antibody (ABIN4900636)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Weissenbacher, Vrekoussis, Roeder, Makrigiannakis, Mayr, Ditsch, Friese, Jeschke, Dian: "Analysis of Epithelial Growth Factor-Receptor (EGFR) Phosphorylation in Uterine Smooth Muscle Tumors: Correlation to Mucin-1 and Galectin-3 Expression." in: International journal of molecular sciences, Vol. 14, Issue 3, pp. 4783-92, 2013 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Dewaele, Floris, Finalet-Ferreiro, Fletcher, Coindre, Guillou, Hogendoorn, Wozniak, Vanspauwen, Schöffski, Marynen, Vandenberghe, Sciot, Debiec-Rychter: "Coactivated platelet-derived growth factor receptor {alpha} and epidermal growth factor receptor are potential therapeutic targets in intimal sarcoma." in: Cancer research, Vol. 70, Issue 18, pp. 7304-14, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN4900636) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr845) (ABIN4900636) Detection of Phospho-EGF R/ErbB1 in EGF-treated A431 Human Cell Line by Flow Cytometr...
Immunocytochemistry (ICC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr845) (ABIN4900636) Phospho-EGF R/ErbB1 (Y845) in A431 Human Cell Line. EGF R/ErbB1 phosphorylated at Y84...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr845) (ABIN4900636) Detection of Human Phospho-EGF R/ErbB1 (Y845) by Western Blot. Western blot shows lys...