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anti-Human EGFR antibody for Immunocytochemistry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Reactivity
Human
2159
749
611
23
14
14
13
10
8
7
4
2
1
1
1
1
1
Host
Mouse
1378
691
63
45
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
93
74
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
1320
568
511
449
421
249
232
184
86
76
70
55
25
11
11
9
8
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2169503
$ 392.86
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.6663565 ABIN2169502 ICC IP WB Mouse IgG1 Log in to see 5E10-G10-E11
0.6663565 ABIN2169500 ICC IP IHC WB Mouse IgG1 Log in to see 3F12-1H7-A10
0.0009962841 ABIN284399 ICC IP ELISA WB Mouse IgG1 kappa pTyr1173 Log in to see 9H2
0.0009962841 ABIN780370 ICC IHC (fro) FACS IP Mouse IgG2b Extracellular Domain Log in to see EGFR1 3
0.0009962841 ABIN1027691 IHC (f) ICC FACS IP PE Mouse IgG2b Extracellular Domain Log in to see EGFR1 4
0.0009962841 ABIN343626 ICC WB Rabbit IgG Log in to see Polyclonal 2
0.0009962841 ABIN1491514 ICC IF ELISA WB Mouse IgG2b, kappa AA 424-605 Log in to see AT2H8
0.0009962841 ABIN189322 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr1173 Log in to see E124
0.0009962841 ABIN2854848 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0009962841 ABIN189316 ICC FACS IF IP WB Rabbit IgG C-Term Log in to see E235
0.0009962841 ABIN189318 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr1068 Log in to see EP38Y
0.0009962841 ABIN457474 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
0.0009962841 ABIN189315 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see E114
0.0009962841 ABIN189319 ICC IF IHC (p) IHC WB Rabbit IgG Tyr1068 Log in to see EP774Y
0.0009962841 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0009962841 ABIN594843 ICC IP ELISA WB Mouse IgG1 AA 1165-1186 Log in to see
0.0009962841 ABIN235070 ICC IP ELISA WB Mouse IgG1 AA 1165-1186 Log in to see 13G8
0.0009962841 ABIN364308 ICC IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal
0.0009962841 ABIN265742 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 29-1 1
0.0009962841 ABIN152028 ICC IF IHC IHC (p) IP PLA WB Rabbit C-Term Log in to see Polyclonal 1

Top referenced anti-EGFR antibody for Immunocytochemistry

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Application / Reactivity Human
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Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 86 Antibodies
ELISA 557 Antibodies
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Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 511 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 25 Antibodies
Immunocytochemistry (ICC) 75 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 190 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Reactivity Human
(2159), (749), (611), (23), (14), (14), (13), (10), (8), (7), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(1378), (691), (63), (45), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(93), (74), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(1320), (568), (511), (449), (421), (249), (232), (184), (86), (76), (70), (55), (25), (11), (11), (9), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity The antibody detects endogenous level of total EGFR.
Cross-Reactivity Monkey
Cross-Reactivity (Details) Does not cross-react with related proteins.
Purification Affinity purified
Immunogen Purified recombinant human EGFR protein fragments expressed in E.coli.
Clone 6H11-2D11-G3
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Subunit structure: Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation. Heterodimer with ERBB2. Interacts with ERRFI1, inhibits dimerization of the kinase domain and autophosphorylation. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts with GRB2, an adapter protein coupling the receptor to downstream signaling pathways. Interacts with GAB2, involved in signaling downstream of EGFR. Interacts with STAT3, mediates EGFR downstream signaling in cell proliferation. Interacts with RIPK1, involved in NF-kappa-B activation. Interacts (autophosphorylated) with CBL, CBLB and CBLC, involved in EGFR ubiquitination and regulation. Interacts with SOCS5, regulates EGFR degradation through TCEB1- and TCEB2-mediated ubiquitination and proteasomal degradation. Interacts with PRMT5, methylates EGFR and enhances interaction with PTPN6. Interacts (phosphorylated) with PTPN6, inhibits EGFR-dependent activation of MAPK/ERK. Interacts with COPG1, essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2, this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts with PCNA, positively regulates PCNA. Interacts with PELP1. Interacts with MUC1. Interacts with AP2M1. Interacts with FER. May interact with EPS8, mediates EPS8 phosphorylation. Interacts (via SH2 domains) with GRB2, NCK1 and NCK2. Interacts with ATX2. Interacts with GAREM. Interacts (ubiquitinated) with ANKRD13A/B/D, the interaction is direct and may regulate EGFR internalization after EGF stimulation. Interacts with GPER1, the interaction occurs in an estrogen-dependent manner.
Subcellular location: Cell membrane, Single-pass type I membrane protein. Endoplasmic reticulum membrane, Single-pass type I membrane protein. Golgi apparatus membrane, Single-pass type I membrane protein. Nucleus membrane, Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note: In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). Isoform 2: Secreted.
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.
Molecular Weight 175 kDa
Gene ID 1956
NCBI Accession NM_005228
UniProt P00533
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Buffer PBS (pH 7.4) containing with 0.02 % sodium azide, 0.1 % BSA and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169503) anti-Epidermal Growth Factor Receptor (EGFR) antibody
Immunocytochemistry (ICC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169503) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 2)
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169503) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 3)
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169503) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 4)
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169503) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 5)