anti-Rat (Rattus) EGFR antibody for Enzyme Immunoassay

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pThr678
148
122
62
59
57
53
53
51
48
48
47
43
38
36
33
30
29
27
26
25
25
25
23
22
21
19
19
18
18
17
16
16
16
16
15
15
15
15
15
15
14
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
2334
849
698
28
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1435
797
67
44
9
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
97
75
66
60
51
49
27
27
26
25
25
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
1362
633
572
529
442
272
245
229
111
84
67
55
35
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN126757
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126756 EIA IP WB Biotin Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN318810 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal 1
1 ABIN5551776 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal
1 ABIN5551788 EIA IHC (p) WB Rabbit IgG AA 1189-1199, pTyr1197 Log in to see Polyclonal
1 ABIN126768 EIA IP WB Biotin Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN265396 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN372504 EIA IF IHC (p) IP WB Sheep IgG Log in to see Polyclonal
1 ABIN5556718 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal

Top referenced anti-EGFR antibody for Enzyme Immunoassay

Similar anti-EGFR Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 23 Antibodies 14 Antibodies 13 Antibodies
Cytometry by Time of Flight (CyTOF) 35 Antibodies 4 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 1350 Antibodies 551 Antibodies 453 Antibodies 19 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies 1 Antibodies
Neutralization (Neut) 14 Antibodies
Luminex Assay (LMNX) 11 Antibodies
Intracellular Staining (ICS) 2 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pThr678
(148), (122), (62), (59), (57), (53), (53), (51), (48), (48), (47), (43), (38), (36), (33), (30), (29), (27), (26), (25), (25), (25), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(2334), (849), (698), (28), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1435), (797), (67), (44), (9), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(97), (75), (66), (60), (51), (49), (27), (27), (26), (25), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(1362), (633), (572), (529), (442), (272), (245), (229), (111), (84), (67), (55), (35), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity This antibody specifically recognizes EGFR phosphorylated at Threonine 678.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Subsequent thiophilic adsorption and size exclusion chromatography
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLH. Remarks: Epitope: Phosphothreonine 678, R K R pT L R R
Clone 3F2
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes ELISA (0.05 μg/mL). Western blot. Immunoprecipitation (1 - 10 μg per 10^6 vanadate treated A431 cells). Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive controlFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate plus PMA. Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl positive control cell lysate correspond to approx. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplicatioins with native proteins such as Immunoprecipitation. Storage: Aliquot and store frozen. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Concentration 0.1 mg/mL
Buffer PBS, 0.09 % Na-Azide, PEG and Sucrose, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pThr678) (ABIN126757) anti-Epidermal Growth Factor Receptor (EGFR) (pThr678) antibody