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anti-Rat (Rattus) EGFR antibody for Enzyme Immunoassay

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 1168-1180
148
122
61
58
54
53
52
50
48
48
48
43
36
35
33
30
29
26
25
25
24
24
23
22
21
19
19
18
18
17
16
16
16
15
15
15
15
15
15
14
13
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2256
846
698
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Rabbit
1386
768
67
43
9
2
1
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
74
66
59
51
49
27
27
26
25
24
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1310
605
559
485
437
272
222
221
109
84
66
55
35
24
19
14
11
8
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118051
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN401341 EIA IHC (p) WB Rabbit IgG AA 1189-1199, pTyr1197 Log in to see Polyclonal 4
1 ABIN401323 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal 4
1 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126756 EIA IP WB Biotin Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126768 EIA IP WB Biotin Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN318810 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal 1
1 ABIN265396 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN372504 EIA IF IHC (p) IP WB Sheep IgG Log in to see Polyclonal

Top referenced anti-EGFR antibody for Enzyme Immunoassay

Similar anti-EGFR Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 142 Antibodies 160 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies 23 Antibodies
Flow Cytometry (FACS) 3 Antibodies 66 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 51 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 220 Antibodies
Immunohistochemistry (IHC) 159 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 453 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 1168-1180
(148), (122), (61), (58), (54), (53), (52), (50), (48), (48), (48), (43), (36), (35), (33), (30), (29), (26), (25), (25), (24), (24), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2256), (846), (698), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1386), (768), (67), (43), (9), (2), (1), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(97), (74), (66), (59), (51), (49), (27), (27), (26), (25), (24), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1310), (605), (559), (485), (437), (272), (222), (221), (109), (84), (66), (55), (35), (24), (19), (14), (11), (8), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN118051) Images
Specificity This antiserum is directed against human epidermal growth factor receptor (EGFR). This antibody can detect EGFR from Human, Mouse and Rat sources. Reactivity of this antibody with EGFR from other species is unknown.
Purification Serum
Immunogen This whole serum was prepared by repeated immunizations with a peptide synthesized using conventional technology. The sequence of the epitope is: NH2-C-S-L-D-N-P-D-Y-Q-Q-D- F-F-P-K-E-COOH and corresponds to amino acids 1168-1180 of the EGFR molecule. Remarks: This maps to a region near the carboxy-terminus which is identical in human, mouse and rat EGFR. The amino terminal Cysteine was synthesized to facilitate carrier coupling.

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN118051) Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors. For example, the epidermal growth factor receptor (EGFR) is mutated and/or overexpressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Overexpression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and ErbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4neu. EGFR can heterodimerize with each of the members of this family.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
NCBI Accession NP_005219
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN118051) Images back to top
Application Notes Anti-EGFR antibody is specifically designed for ELISA, Immunoblotting andImmunoprecipitation. Reactivity in other assays is likely, but has not been determined. Recognition of EGFR is independent of the phosphorylation status at tyrosine 1173. No reaction is observed against ErbB-2, ErbB-3 or ErbB-4. A431 cells, keratinocytes innormal epidermis, or placenta are typically used as positive control sources. The antigen istypically localized in the cell membrane. For more details see Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Western blotting: Good results are also achieved on PVDF membranes blocked with 5%lowfat milk diluted in TTBS for 1 hour at room temperature. Also, dilute the primaryantibody and secondary in 5% lowfat milk in TTBS. Anti-EGFR can be diluted up to 1: 10,000for immunoblot depending on the cell line and the amount of EGFR in a particular lysate. Immunoprecipitation: Use approximately 10 μl of the antibody. The immunoprecipitationmix contains the antibody, 25 μl of Protein A-agarose beads and 1. 0 ml of lysate (lysatecontains approximately 1. 0 mg of total protein). This mixture is rotated overnight at 4°Cand then washed 3 times with lysis buffer (used to prepare the lysate). The resulting beadcomplex is dissolved in 20-30 μl of 3X SDS-PAGE sample buffer and approximately 15 μl isloaded per lane on an 8% polyacrylamide gel. Recommnded dilutions: ELISA 1: 10,000 - 1: 50,000Western blot 1: 1,000-1: 10,000Immunoprecipitation: 10 µl
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN118051) Images back to top
Format Liquid
Concentration 85 mg/mL (by refractometry)
Handling Advice . Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.

References for anti-EGFR Antibody (ABIN118051)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Scambia, Catozzi, Panici, Ferrandina, Coronetta, Barozzi, Baiocchi, Uccelli, Piffanelli, Mancuso: "Expression of ras oncogene p21 protein in normal and neoplastic ovarian tissues: correlation with histopathologic features and receptors for estrogen, progesterone, and epidermal growth factor." in: American journal of obstetrics and gynecology, Vol. 168, Issue 1 Pt 1, pp. 71-8, 1993

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN118051) back to top
Supplier Images
 image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1168-1180) (ABIN118051) Combined immunoprecipitation and immunoblot of a human cell lysate using anti-EGFR an...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1168-1180) (ABIN118051) Western blot using anti-EGFR antibody shows detection of a band at ~170 kDa correspon...