|Application / Reactivity||Human||Mouse (Murine)||Rat (Meriones)|
|Western Blotting (WB)||1109 Antibodies||444 Antibodies||1 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Neutralization (Neut)||3 Antibodies|
|Luminex Assay (LMNX)||11 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies||2 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Inhibition Assay (InhA)||2 Antibodies|
|Indirect Immunofluorescence Assay (IFA)||2 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||215 Antibodies||36 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||255 Antibodies|
|Antigen||Epidermal Growth Factor Receptor (EGFR) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Meriones) Alternatives|
|Conjugate||This EGFR antibody is un-conjugated Alternatives|
Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-EGFR AntibodyTarget Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN2801937) Images
Target Details EGFRProduct Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN2801937) Images back to top
|Alternative Name||EGFR (EGFR Antibody Abstract)|
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Synonyms: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
|Molecular Weight||134277 Da|
|Research Area||Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN2801937) Images back to top
|Application Notes||WB - 1:1000|
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN2801937) Images back to top
|Buffer||Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
References for anti-EGFR Antibody (ABIN2801937)Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
|Product cited in:||
Reiter, Threadgill, Eley et al.: "Comparative genomic sequence analysis and isolation of human and mouse alternative EGFR transcripts encoding truncated receptor isoforms." in: Genomics, Vol. 71, Issue 1, pp. 1-20, 2001 (PubMed).
Ilekis, Gariti, Niederberger et al.: "Expression of a truncated epidermal growth factor receptor-like protein (TEGFR) in ovarian cancer." in: Gynecologic oncology, Vol. 65, Issue 1, pp. 36-41, 1997 (PubMed).
Reiter, Maihle: "A 1.8 kb alternative transcript from the human epidermal growth factor receptor gene encodes a truncated form of the receptor." in: Nucleic acids research, Vol. 24, Issue 20, pp. 4050-6, 1996 (PubMed).
Ilekis, Stark, Scoccia: "Possible role of variant RNA transcripts in the regulation of epidermal growth factor receptor expression in human placenta." in: Molecular reproduction and development, Vol. 41, Issue 2, pp. 149-56, 1995 (PubMed).
Ullrich, Coussens, Hayflick et al.: "Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells." in: Nature, Vol. 309, Issue 5967, pp. 418-25, 1984 (PubMed).
ImagesProduct Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN2801937) back to top