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anti-Cow (Bovine) IL1B antibody for ELISA

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
AA 109-251
30
28
26
21
15
15
15
14
12
11
10
7
7
7
6
6
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
514
174
123
37
26
19
16
13
12
11
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
382
347
17
16
8
7
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
94
45
33
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
505
409
165
124
109
72
58
53
37
29
24
24
14
14
13
7
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN1859381
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN326595 FACS IHC (fro) ELISA Mouse IgG1 Log in to see ID4
1 ABIN2474897 ELISA Rabbit IgG Log in to see Polyclonal 4
1 ABIN2474903 ELISA WB Rabbit IgG Log in to see Polyclonal 4
1 ABIN2474896 ELISA Biotin Rabbit IgG Log in to see Polyclonal 4
1 ABIN212937 ELISA Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2609639 ELISA WB Rabbit IgG AA 109-251 Log in to see Polyclonal
1 ABIN212757 ELISA Biotin Rabbit Log in to see Polyclonal
1 ABIN2289940 ELISA Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2474895 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 1D4 4
1 ABIN217582 ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN638922 ELISA Biotin Rabbit IgG Log in to see
1 ABIN2290051 ELISA Rabbit Log in to see Polyclonal
1 ABIN638923 ELISA Rabbit IgG Log in to see
1 ABIN2935278 ELISA IF/ICC IHC WB Rabbit AA 1-266 Log in to see Polyclonal
1 ABIN2935275 ELISA IF/ICC IHC WB Rabbit AA 109-251 Log in to see Polyclonal

Top referenced anti-IL1B antibody for ELISA

Similar anti-IL1B Antibodies

Application / Reactivity Cow (Bovine)
ELISA 16 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 7 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 109-251
(30), (28), (26), (21), (15), (15), (15), (14), (12), (11), (10), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(514), (174), (123), (37), (26), (19), (16), (13), (12), (11), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(382), (347), (17), (16), (8), (7), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(94), (45), (33), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(505), (409), (165), (124), (109), (72), (58), (53), (37), (29), (24), (24), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IK PRSAHYSFQS NVKYNFMRVI HQECILNDAL NQSIIRDMSG PYLTATTLNN LEEAVKFDMV AYVSEEDSQL PVTLRISKTQ LFVSAQNEDE PVLLKEMPET PKIIKDETNL LFFWEKHGSM DYFKSVAHPK LFIATKQEKL V
Specificity It has been selected for its ability to recognize IL1b in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL1b (AA 109-251)
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 1 Beta (IL1b) (IL1B Antibody Abstract)
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IL1b (Ile109~Val251) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (AA 109-251) (ABIN1859381) anti-Interleukin 1, beta (IL1B) (AA 109-251) antibody