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|Application / Reactivity||Sheep (Ovine)||Goat||Cow (Bovine)|
|ELISA||25 Antibodies||3 Antibodies||18 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||1 Antibodies||3 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies||3 Antibodies||3 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||2 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunohistochemistry (IHC)||4 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||26 Antibodies||2 Antibodies|
|Antigen||Interleukin 1, beta (IL1B) Antibodies|
|Reactivity||Cow (Bovine), Goat, Sheep (Ovine) Alternatives|
|Conjugate||This IL1B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|1 reference available|
|Supplier||Log in to see|
Product Details anti-IL1B AntibodyTarget Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN120889) Images
|Immunogen||Recombinant ovine IL-1 Beta|
Target Details IL1BProduct Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN120889) Images back to top
|Alternative Name||Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)|
|Background||Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2|
|Pathways||NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity|
Application DetailsProduct Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN120889) Images back to top
ELISA: 5 μg/mL as coating antibody. Flow Cytometry: 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN120889) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
|Expiry Date||12 months|
References for anti-IL1B Antibody (ABIN120889)Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Rothel, Hurst, Seow, Pépin, Berthon, Corner, Wood: "Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry." in: Veterinary immunology and immunopathology, Vol. 57, Issue 3-4, pp. 267-78, 1997