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anti-Cow (Bovine) IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
AA 109-251
33
26
20
18
17
16
13
12
9
9
7
7
6
6
5
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
503
170
121
38
23
19
16
11
11
10
8
7
6
3
3
2
2
1
Host
Rabbit
398
333
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
49
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
498
401
147
123
111
70
63
46
30
29
24
21
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1859381
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011739905 ABIN2474903 ELISA WB Rabbit IgG Log in to see Polyclonal 4
0.0011739905 ABIN2474895 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 1D4 4

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Cow (Bovine)
ELISA 15 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 3 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 109-251
(33), (26), (20), (18), (17), (16), (13), (12), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(503), (170), (121), (38), (23), (19), (16), (11), (11), (10), (8), (7), (6), (3), (3), (2), (2), (1)
Host Rabbit
(398), (333), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(100), (49), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(498), (401), (147), (123), (111), (70), (63), (46), (30), (29), (24), (21), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IK PRSAHYSFQS NVKYNFMRVI HQECILNDAL NQSIIRDMSG PYLTATTLNN LEEAVKFDMV AYVSEEDSQL PVTLRISKTQ LFVSAQNEDE PVLLKEMPET PKIIKDETNL LFFWEKHGSM DYFKSVAHPK LFIATKQEKL V
Specificity It has been selected for its ability to recognize IL1b in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL1b (AA 109-251)
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 1 Beta (IL1b) (IL1B Antibody Abstract)
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IL1b (Ile109~Val251) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (AA 109-251) (ABIN1859381) anti-Interleukin 1, beta (IL1B) (AA 109-251) antibody