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anti-Dog (Canine) IL1B antibody for Immunohistochemistry (Frozen Sections)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
N-Term
30
29
26
20
15
15
15
14
12
11
11
7
7
7
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Primate
518
179
125
37
26
20
16
13
12
12
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
385
351
17
16
8
7
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
94
45
33
22
14
12
11
11
9
9
8
8
8
8
8
8
8
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
508
405
165
132
110
72
58
52
38
30
24
24
14
14
12
7
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN799612
$ 1,644.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3201448 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
1 ABIN799831 IHC (fro) IHC (p) IP ELISA WB Rabbit N-Term Log in to see Polyclonal

Similar anti-IL1B Antibodies

Application / Reactivity Primate Human
Electron Microscopy (EM) 1 Antibodies 1 Antibodies
ELISA 13 Antibodies 217 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Neutralization (Neut) 4 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 13 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(30), (29), (26), (20), (15), (15), (15), (14), (12), (11), (11), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Primate
(518), (179), (125), (37), (26), (20), (16), (13), (12), (12), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(385), (351), (17), (16), (8), (7), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(94), (45), (33), (22), (14), (12), (11), (11), (9), (9), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(508), (405), (165), (132), (110), (72), (58), (52), (38), (30), (24), (24), (14), (14), (12), (7), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).
Purification Purified IgG
Immunogen Recombinant human IL1B / IL1 Beta.

Type of Immunogen: Recombinant protein

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta
Gene ID 3553
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Approved: ELISA (1:500 - 1:2000), IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), RIA (1:5000 - 1:10000), WB (1:100 - 1:200)

Usage: This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C. Minimize freezing and thawing.

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
 image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN799612) anti-Interleukin 1, beta (IL1B) (N-Term) antibody