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anti-Dog (Canine) IL1B antibody for Immunohistochemistry (Frozen Sections)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
AA 103-260
33
27
25
23
18
16
13
12
9
9
8
7
7
6
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine)
519
166
121
38
25
20
17
12
12
10
8
7
6
3
3
2
2
2
1
1
Host
Rabbit
404
342
17
16
8
8
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
47
35
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
503
415
154
125
112
71
68
47
37
29
27
24
13
12
7
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1859379
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3201448 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see

Similar anti-IL1B Antibodies

Application / Reactivity Dog (Canine)
Electron Microscopy (EM) 1 Antibodies
ELISA 19 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Neutralization (Neut) 6 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 23 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 103-260
(33), (27), (25), (23), (18), (16), (13), (12), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine)
(519), (166), (121), (38), (25), (20), (17), (12), (12), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(404), (342), (17), (16), (8), (8), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(100), (47), (35), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(503), (415), (154), (125), (112), (71), (68), (47), (37), (29), (27), (24), (13), (12), (7), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Sequence MGHHHHHHSG S- CKTDADNF MSDAAMQSVD CKLQDISHKY LVLSNSYELR ALHLNGENVN KQVVFHMSFV HGDESNNKIP VVLGIKQKNL YLSCVMKDGK PTLQLEKVDP KVYPKRKMEK RFVFNKIEIK NTVEFESSQY PNWYISTSQV EGMPVFLGNT RGGQDITDFT
Specificity It has been selected for its ability to recognize IL1b in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL1b (AA 103-260)
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 1 Beta (IL1b) (IL1B Antibody Abstract)
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IL1b (Cys103~Thr260) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (AA 103-260) (ABIN1859379) anti-Interleukin 1, beta (IL1B) (AA 103-260) antibody