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anti-Human IL1B antibody for Immunohistochemistry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
N-Term
33
26
25
23
18
16
13
12
9
9
8
7
7
6
5
5
5
5
5
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
518
166
121
38
26
20
17
12
12
10
8
7
6
3
3
2
2
2
1
1
Host
Rabbit
404
342
17
16
8
8
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
47
35
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
503
416
153
125
113
71
69
47
38
29
27
24
13
12
7
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2785654
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.514159 ABIN2785655 IHC WB Rabbit N-Term Log in to see Polyclonal 1
8.514159 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
8.514159 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
8.514159 ABIN446969 IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal 3
8.514159 ABIN1536091 IHC ELISA Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
8.514159 ABIN1536090 IHC ELISA WB Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
8.514159 ABIN2706371 IHC WB Rabbit Center Log in to see Polyclonal
8.514159 ABIN4324389 IF IHC WB Mouse IgG1 Log in to see 3E1
8.514159 ABIN3031434 FACS IHC ELISA WB Rabbit Ig Fraction AA 148-174 Log in to see Polyclonal
8.514159 ABIN3031433 FACS IHC ELISA WB Rabbit Ig Fraction AA 177-206 Log in to see Polyclonal
8.514159 ABIN2428273 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
8.514159 ABIN2487916 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
8.514159 ABIN1845349 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
1 ABIN1582289 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 3
1 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
1 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
1 ABIN3185165 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1498871 ICC IF IHC ELISA WB Mouse IgG1 Log in to see 3A6
1 ABIN964777 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
1 ABIN103012 FACS IP IHC Neut RIA ELISA WB Rabbit IgG Cleavage Site, N-Term, AA 153 Log in to see Polyclonal

Top referenced anti-IL1B antibody for Immunohistochemistry

Similar anti-IL1B Antibodies

Application / Reactivity Human
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 225 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 96 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(33), (26), (25), (23), (18), (16), (13), (12), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(518), (166), (121), (38), (26), (20), (17), (12), (12), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(404), (342), (17), (16), (8), (8), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(100), (47), (35), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(503), (416), (153), (125), (113), (71), (69), (47), (38), (29), (27), (24), (13), (12), (7), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785654) Images
Blocking Peptide Blocking peptide for this product available: ABIN978485
Sequence MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL
Predicted Reactivity Human : 100 %, Dog : 90 %, Rabbit : 90 %, Horse : 80 %, Mouse : 70 %
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN2785654) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Molecular Weight 17
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN2785654) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 269 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN2785654) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN2785654)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Wang, Jiang, Li, He, Zhao, Yang, Zhu, Yin, Li, Wang, Liu, Shang, Yang, Wang, Zhen, Zhang, Yu, Zhong, Ye, Huang, Yuan: "Shigella flexneri T3SS effector IpaH4.5 modulates the host inflammatory response via interaction with NF-κB p65 protein." in: Cellular microbiology, Vol. 15, Issue 3, pp. 474-85, 2013

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785654) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785654) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785654) Sample Type: Human Macrophange Cells
Green: primary
Red: p...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785654) WB Suggested Anti-IL1B
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785654) WB Suggested Anti-IL1B Antibody
Positive Control: Lane 1:841 µg rat brain extr...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785654) Human Macrophage