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anti-Human IL1B antibody for Immunohistochemistry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
N-Term
33
25
20
18
17
16
13
12
9
9
7
7
6
6
5
5
5
5
5
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
502
170
121
38
23
20
16
11
11
10
8
7
6
3
3
2
2
1
Host
Rabbit
398
333
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
49
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
498
402
146
123
112
70
64
46
31
29
24
21
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2785655
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22915025 ABIN2785654 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.22915025 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
0.22915025 ABIN1536090 IHC ELISA WB Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
0.22915025 ABIN1536091 IHC ELISA Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
0.22915025 ABIN2706371 IHC WB Rabbit Center Log in to see Polyclonal
0.22915025 ABIN2428273 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN2487916 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.22915025 ABIN1845349 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
0.0011319318 ABIN1582289 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 3
0.0011319318 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0011319318 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0011319318 ABIN288401 IHC ELISA WB Biotin Goat IgG Log in to see Polyclonal
0.0011319318 ABIN284570 FACS IHC ELISA WB Mouse IgG1 Log in to see 9627
0.0011319318 ABIN964777 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0011319318 ABIN1498871 ICC IF IHC ELISA WB Mouse IgG1 Log in to see 3A6
0.0011319318 ABIN1102516 FACS IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
0.0011319318 ABIN103012 FACS IP IHC Neut RIA ELISA WB Rabbit IgG Cleavage Site, N-Term, AA 153 Log in to see Polyclonal
0.0011319318 ABIN269770 IEM IHC (p) IP IHC ELISA WB Rabbit IgG Ala117, N-Term, Cleavage Site Log in to see Polyclonal
0.0011319318 ABIN1873201 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN284559 IHC ELISA Mouse IgG2b Log in to see 1E6

Top referenced anti-IL1B antibody for Immunohistochemistry

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Application / Reactivity Human
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Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA 222 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 97 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
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Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 19 Antibodies
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Immunohistochemistry (IHC) 120 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(33), (25), (20), (18), (17), (16), (13), (12), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(502), (170), (121), (38), (23), (20), (16), (11), (11), (10), (8), (7), (6), (3), (3), (2), (2), (1)
Host Rabbit
(398), (333), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(100), (49), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(498), (402), (146), (123), (112), (70), (64), (46), (31), (29), (24), (21), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785655) Images
Blocking Peptide Blocking peptide for this product available: ABIN978486
Sequence DLDLCPLDGG IQLRISDHHY SKGFRQAASV VVAMDKLRKM LVPCPQTFQE
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN2785655) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN2785655) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 153 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN2785655) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN2785655)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Piccini, Carta, Tassi, Lasiglié, Fossati, Rubartelli: "ATP is released by monocytes stimulated with pathogen-sensing receptor ligands and induces IL-1beta and IL-18 secretion in an autocrine way." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8067-72, 2008

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785655) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785655) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785655) Sample Type: Human Macrophange Cells
Green: primary
Red: p...