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anti-Monkey IL1B antibody for Immunoelectron Microscopy

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
Ala117, N-Term, Cleavage Site
33
26
20
18
17
16
13
12
9
9
7
7
6
6
5
5
5
5
5
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Monkey
503
170
121
38
23
20
16
11
11
10
8
7
6
3
3
2
2
1
Host
Rabbit
399
333
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
49
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
499
402
147
123
112
70
64
46
31
29
24
21
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN269770
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Similar anti-IL1B Antibodies

Application / Reactivity Monkey Human
ELISA 1 Antibodies 222 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 15 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 5 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope Ala117, N-Term, Cleavage Site
(33), (26), (20), (18), (17), (16), (13), (12), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Monkey
(503), (170), (121), (38), (23), (20), (16), (11), (11), (10), (8), (7), (6), (3), (3), (2), (2), (1)
Host Rabbit
(399), (333), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(100), (49), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(499), (402), (147), (123), (112), (70), (64), (46), (31), (29), (24), (21), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW human IL-1 beta and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1 beta in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1 beta exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, a 1:100 to 1:200 dilution of this antiserum detected the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1 beta precursor in lysates. This antibody is also useful for neutralization of human and primate IL-1 beta activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1 beta. It will neutralize primate IL-1 beta. For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for analysis. Caution should be exhibited as the F (c) domain of the rabbit IgG molecule may interact with cells non-specifically.
No Cross-Reactivity Mouse (Murine), Rabbit, Rat (Rattus)
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Immunogen Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-1 beta/il-1F2 (IL1B Antibody Abstract)
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates Tcells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarilyreleased from stimulated macrophages and monocytes, but also is released fromseveral other cell types, and is thought to play a key role in inflammatory and immuneresponses. The two closely related agents, interleukin1 alpha (IL1 alpha) andinterleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identicalbiological responses and share 25% homology in their amino acid sequence. Alternate Names: anti-Catabolin antibody, anti-H1 antibody, anti-Hematopoietin 1 antibody, anti-IFN betainducing factor antibody, anti-IL 1B antibody, anti-IL1B antibody, anti-IL1F2 antibody,anti-Interleukin 1 beta precursor antibody, anti-LAF antibody, anti-OAF antibody,anti-Osteoclast activating factor antibody, anti-Preinterleukin beta antibody, anti-Prointerleukin 1 beta antibody.
Gene Symbol: IL1B
Gene ID 3553, 16176, 24494
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes This antibody recognizes the 17,000 MW mature IL-1Beta . For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells . PBMC are stimulated for 24 hours with 1% serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG - reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation - stain intracellular IL-1Beta . For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of murine, rat or rabbit IL-1Beta . For neutralization, it is recommended - incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically. Electron Microscopy was reported in scientific literature.
Recommended dilutions: Electron Microscopy, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunohistochemistry-Paraffin 1:10-1:2000, Immunoprecipitation 1:400-1:800, Western Blot 1:1000
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.02 M sodium chloride, pH 7.2
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (Ala117) (ABIN269770) anti-Interleukin 1, beta (IL1B) (Ala117), (Cleavage Site), (N-Term) antibody