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anti-Pig (Porcine) IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
N-Term
30
28
26
20
15
15
15
14
12
11
10
7
7
7
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Bacteria, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
513
173
122
37
25
20
15
12
12
11
8
7
6
4
4
2
2
2
1
Host
Rabbit
382
347
17
16
8
7
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
94
45
33
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
505
409
164
124
109
72
59
52
37
29
24
23
14
14
13
7
5
4
4
3
3
3
3
2
1
1
1
1
1
1
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Catalog No. ABIN269770
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0741818 ABIN1859384 ICC IHC IP WB Rabbit IgG AA 95-262 Log in to see Polyclonal
1 ABIN1582289 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 3
1 ABIN119945 WB Mouse IgG1 Log in to see DF8
1 ABIN2474894 WB Mouse IgG1 Log in to see DF8 1
1 ABIN2623961 ELISA WB Biotin Rabbit IgG AA 95-262 Log in to see Polyclonal
1 ABIN2609649 ELISA WB FITC Rabbit IgG AA 95-262 Log in to see Polyclonal
1 ABIN326372 WB Mouse IgG1 Log in to see DF8
1 ABIN2609636 ELISA WB Rabbit IgG AA 95-262 Log in to see Polyclonal
1 ABIN218053 WB Rabbit Log in to see Polyclonal
1 ABIN217734 WB Mouse IgG1 Log in to see
1 ABIN2298243 WB Mouse IgG1 Log in to see 6A420
1 ABIN3201454 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
1 ABIN2298254 WB Rabbit IgG Log in to see Polyclonal
1 ABIN643916 WB Mouse IgG1 Log in to see
1 ABIN2935276 ELISA IF/ICC IHC WB Rabbit AA 95-262 Log in to see Polyclonal

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Rat (Rattus) Primate
Blocking Antibody (Inhibition) 3 Antibodies
Electron Microscopy (EM) 1 Antibodies 1 Antibodies
ELISA 61 Antibodies 13 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Neutralization (Neut) 15 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 97 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(30), (28), (26), (20), (15), (15), (15), (14), (12), (11), (10), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bacteria, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
(513), (173), (122), (37), (25), (20), (15), (12), (12), (11), (8), (7), (6), (4), (4), (2), (2), (2), (1)
Host Rabbit
(382), (347), (17), (16), (8), (7), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(94), (45), (33), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(505), (409), (164), (124), (109), (72), (59), (52), (37), (29), (24), (23), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN269770) Images
Specificity Detects IL-1 beta and does not cross react with IL-1 alpha. Weakly detects the non-denatured 31 kDa precursor molecule but strongly detects all of the 17 kDa mature molecule. Additionally this IL-1 beta antibody will bind the denatured 31 kDa precursor molecule with greater affinity than the native form, but will often detect past the 35 kDa mark. This IL-1 beta antibody has also been shown to be useful in neutralization of human and primate IL-1 beta activity in bioassays.
Purification IgG purified
Immunogen Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN269770) Images back to top
Antigen
Alternative Name IL-1 beta/il-1F2 (IL1B Antibody Abstract)
Background Gene Symbol: IL1B
Gene ID 3553
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN269770) Images back to top
Application Notes Western Blot 1:1000, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Electron MicroscopyAnti-Human IL-1B has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17,000 MW mature IL-1B. For immunoblots, typically, IL-1B is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1B. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1B activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1B. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN269770) Images back to top
Format Liquid
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN269770)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Ahmad, Chung, Tang, Hockman, Liu, Khan, Huo, Billings, Amar, Remaley, Manganiello: "Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue." in: Scientific reports, Vol. 6, pp. 28056, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Su, Luo, Zhu, Fu, Zhao, Chen, Zhang, Ren, Yu, Yang, Wu, Feng, Li, Chen, Wang: "Interleukin-1β/Iinterleukin-1 receptor-associated kinase 1 inflammatory signaling contributes to persistent Gankyrin activation during hepatocarcinogenesis." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 2, pp. 585-97, 2015 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Bimpis, Papalois, Voumvourakis, Oláh, Tiszlavicz, Liapi: "Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G." in: Brain research, Vol. 1615, pp. 98-105, 2015 (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Arendt, McCready, Keller, Baker, Naber, Seewaldt, Kuperwasser: "Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis." in: Cancer research, Vol. 73, Issue 19, pp. 6080-93, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Paino, Ahlstrand, Nuutila, Navickaite, Lahti, Tuominen, Välimaa, Lamminmäki, Pöllänen, Ihalin: "Identification of a novel bacterial outer membrane interleukin-1Β-binding protein from Aggregatibacter actinomycetemcomitans." in: PLoS ONE, Vol. 8, Issue 7, pp. e70509, 2013 (Sample species: Bacteria). Further details: Western Blotting

Paino, Lohermaa, Sormunen, Tuominen, Korhonen, Pöllänen, Ihalin: "Interleukin-1β is internalised by viable Aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids." in: Cytokine, Vol. 60, Issue 2, pp. 565-74, 2012 (Sample species: Bacteria). Further details: Immunoelectron Microscopy,Immunohistochemistry

Petrovski, Ayna, Majai, Hodrea, Benko, Mádi, Fésüs: "Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages." in: Autophagy, Vol. 7, Issue 3, pp. 321-30, 2011 (Sample species: Human). Further details: Western Blotting

Paino, Tuominen, Jääskeläinen, Alanko, Nuutila, Asikainen, Pelliniemi, Pöllänen, Chen, Ihalin: "Trimeric form of intracellular ATP synthase subunit β of Aggregatibacter actinomycetemcomitans binds human interleukin-1β." in: PLoS ONE, Vol. 6, Issue 4, pp. e18929, 2011

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN269770) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) anti-Interleukin 1, beta (IL1B) (N-Term) antibody
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Lane 1: Human IL-1Beta. Load: ...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Antibody was used at a 1:200 d...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - pro-IL-1beta in glioblastoma c...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Analysis of IL-1 beta in Rhesu...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Analysis of: Human IL1...
Immunofluorescence (IF) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Trab...