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anti-Rat (Rattus) IL1B antibody for Enzyme Immunoassay

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Mouse (Murine), Rat (Rattus)
477
165
116
38
20
20
16
11
10
8
8
7
6
3
3
2
2
1
Host
Rabbit
390
312
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
98
49
33
21
12
10
9
9
8
8
8
8
8
8
8
8
7
7
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Radioimmunoassay (RIA), Western Blotting (WB)
478
392
138
110
101
67
63
44
29
28
24
17
14
12
12
7
5
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN616079
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008806384 ABIN115953 EIA Func WB Rabbit Log in to see Polyclonal
0.0008806384 ABIN115954 EIA Func WB Rabbit Log in to see Polyclonal
0.0008806384 ABIN115952 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008806384 ABIN115951 EIA WB Biotin Rabbit Log in to see Polyclonal

Top referenced anti-IL1B antibody for Enzyme Immunoassay

Similar anti-IL1B Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies 2 Antibodies
ELISA 57 Antibodies 80 Antibodies
ELISA (Capture) 2 Antibodies 2 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Neutralization (Neut) 16 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 86 Antibodies
ELISA (Detection) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(477), (165), (116), (38), (20), (20), (16), (11), (10), (8), (8), (7), (6), (3), (3), (2), (2), (1)
Host Rabbit
(390), (312), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(98), (49), (33), (21), (12), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (7), (7), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Radioimmunoassay (RIA), Western Blotting (WB)
(478), (392), (138), (110), (101), (67), (63), (44), (29), (28), (24), (17), (14), (12), (12), (7), (5), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN616079) Images
Specificity This antibody is primarily directed against mature, 17,000 MW Mouse IL1-beta and is useful in determining its presence in various assays. The antibody does not recognize Human IL1-beta or Mouse IL1-alpha. In ELISA formats and other immunoreactive assays, reactivity occurs with Rat IL1-beta. This antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW Mouse IL1-beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in Immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1-beta precursor molecule. Denatured 31,000 precursor IL1-beta will be recognized by this antibody.
Purification DEAE Fractionation
Immunogen Recombinant Mouse IL-1 beta produced in E. coli Genename: IL1b Remarks: The MW of the recombinant Mouse IL-1 beta is 17 kDa

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN616079) Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background IL-1 beta (also known as Interleukin-1 beta, IL1-beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 16176
NCBI Accession NP_032387
UniProt P10749
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN616079) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN616079) Images back to top
Reconstitution Restore with 0.1 mL of deionized water (or equivalent)
Concentration 1.0 mg/mL (UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, None, None
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C or -20 °C. Following reconstitution store at -20 °C.

References for anti-IL1B Antibody (ABIN616079)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Huang, Newton, Rutledge, Horuk, Matthew, Covington, Lin: "Characterization of murine IL-1 beta. Isolation, expression, and purification." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3838-43, 1988

Telford, Macchia, Massone, Carinci, Palla, Melli: "The murine interleukin 1 beta gene: structure and evolution." in: Nucleic acids research, Vol. 14, Issue 24, pp. 9955-63, 1987

Gray, Glaister, Chen, Goeddel, Pennica: "Two interleukin 1 genes in the mouse: cloning and expression of the cDNA for murine interleukin 1 beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 11, pp. 3644-8, 1986