|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||12 Antibodies||23 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||8 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||5 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||8 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|SDS-PAGE (SDS)||1 Antibodies|
|Western Blotting (WB)||39 Antibodies|
|Antigen||Myeloid Differentiation Primary Response Gene (88) (MYD88) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MYD88 antibody is un-conjugated Alternatives|
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-MYD88 AntibodyTarget Details MYD88 Application Details Handling References for anti-MYD88 Antibody (ABIN493143) Images
|Specificity||This antibody labels MyD88 at 59 kDa.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Immunogen||Synthetic peptide was selected from the unique amino-terminal end of MYD88|
Target Details MYD88Product Details anti-MYD88 Antibody Application Details Handling References for anti-MYD88 Antibody (ABIN493143) Images back to top
|Alternative Name||MYD88 (MYD88 Antibody Abstract)|
|Background||The mammalian host defense system is essentially regulated by Toll-like receptor (TLR) family. Toll-like receptors (TLRs) play an important role in recognition of microbial components and induction of innate immunity. At least 10 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens. The Toll signaling to NF-Kb starts form conserved Toll-IL-1-resistance (TIR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM). Most of the TLRs are dependent on the expression of adapter molecules MyD88 for all their function, the TL3 and TLR 4 are unique in their ability to mediate both MyD88-dependent and independent reposes (1). The MyD88 independent pathway involves induction of a DC maturation and induction of type 1 interferon (IFN-B). Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly up-regulated during differentiation of the myeloleukemic cell line M1. The murine MyD88 is 53 amino acids larger than human MyD88 and maps to chromosome 3p21.3-p22. The over expression of death region in MyD88 lead to transcriptional activation of IL-8 promoter. The microbial components trigger the activation of two downstream signaling pathways of TLRs, MyD88- and/or TRIF-dependent pathways leading to activation of NF-kappaB. The MyD88 transduces a core set of Toll-like receptor (TLR)-induced signals, microbial-induced host responses are divided broadly into the MyD88-dependent and MyD88-independent pathways. The specific pathogen induced early host responses are generally mediated by MyD88 dependent pathways. Inhaled endotoxins induce an acute inflammatory response in the airways mediated through Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88). The endotoxin-induced acute bronchoconstriction, vascular damage resulting in protein leak, Th1 cytokine and chemokine secretion and neutrophil recruitment in the airways are abrogated in mice deficient for either TIRAP or MyD88, but not in TRIF deficient mice (2). The bronchoconstriction and acute inflammatory lung pathology mediated by TLR-4 to inhaled endotoxins critically dependent on the expression and co-operative role of adaptor proteins, TIRAP and MyD88 (3). The MyD88 mediated post-TLR signaling is also involved in a number of allergents and endotoxins.Synonyms: Myeloid differentiation primary response protein MyD88|
|Pathways||NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MYD88 Antibody Target Details MYD88 Handling References for anti-MYD88 Antibody (ABIN493143) Images back to top
Immunohistochemistry on frozen sections. Dot blot: 1: 20,000. ELISA: 1: 20,000. Western blot: 1: 500-1: 750.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MYD88 Antibody Target Details MYD88 Application Details References for anti-MYD88 Antibody (ABIN493143) Images back to top
|Concentration||0.68 - 0.98 mg/mL|
|Buffer||Stabilization buffer with preservatives|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.|
References for anti-MYD88 Antibody (ABIN493143)Product Details anti-MYD88 Antibody Target Details MYD88 Application Details Handling Images back to top
Togbe, Aurore, Noulin et al.: "Nonredundant roles of TIRAP and MyD88 in airway response to endotoxin, independent of TRIF, IL-1 and IL-18 pathways." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 86, Issue 11, pp. 1126-35, 2006 (PubMed).
Fitzgerald, Rowe, Barnes et al.: "LPS-TLR4 signaling to IRF-3/7 and NF-kappaB involves the toll adapters TRAM and TRIF." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1043-55, 2003 (PubMed).
Oshiumi, Matsumoto, Funami et al.: "TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction." in: Nature immunology, Vol. 4, Issue 2, pp. 161-7, 2003 (PubMed).