anti-Rabbit TGFA antibody for Western Blotting

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • TGFA
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TGFAA
  • wa-1
  • wa1
  • TFGA
  • TGF alpha
  • transforming growth factor, alpha
  • transforming growth factor alpha
  • transforming growth factor-alpha
  • tgfa
  • TGFA
  • LOC100009482
  • LOC100121300
  • Tgfa
  • TGF-A
  • LOC100009150
  • LOC100358936
Epitope
AA 24-98
101
77
16
6
5
5
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Rabbit
344
120
117
94
72
37
6
3
2
2
Host
Guinea Pig
206
139
17
4
Clonality
Polyclonal
Conjugate
This TGFA antibody is un-conjugated
26
13
10
9
8
8
7
7
7
7
7
7
7
7
7
7
7
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
180
174
163
159
146
115
113
65
62
18
14
13
8
4
4
2
2
2
1
1
Options
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Catalog No. ABIN1860726
$ 342.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2707168 WB Rabbit Center Log in to see Polyclonal
1 ABIN4358971 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 kappa AA 34-43 Log in to see P-T1
1 ABIN1585841 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN4358984 WB PerCP Mouse IgG1 kappa AA 34-43 Log in to see TG86
1 ABIN4358967 WB PerCP Mouse IgG1 kappa AA 34-43 Log in to see P-T1
1 ABIN4358970 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 kappa AA 34-43 Log in to see P-T1
1 ABIN4358935 WB PerCP Mouse IgM kappa AA 34-43 Log in to see 1E8-G6
1 ABIN4358993 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM Log in to see TG86-P-T1
1 ABIN4359001 WB PerCP Mouse IgM Log in to see TG86-P-T1
1 ABIN4359004 FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM Log in to see TG86-P-T1
1 ABIN4359003 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM Log in to see TG86-P-T1
1 ABIN4359005 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM Log in to see TG86-P-T1
1 ABIN4358991 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM Log in to see TG86-P-T1
1 ABIN4358990 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM Log in to see TG86-P-T1
1 ABIN4358992 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM Log in to see TG86-P-T1
1 ABIN4358996 FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM Log in to see TG86-P-T1
1 ABIN4358997 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM Log in to see TG86-P-T1
1 ABIN4358998 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM Log in to see TG86-P-T1
1 ABIN4359000 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM Log in to see TG86-P-T1
1 ABIN2574142 WB Rabbit Internal Region Log in to see Polyclonal

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Epitope AA 24-98
(101), (77), (16), (6), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(344), (120), (117), (94), (72), (37), (6), (3), (2), (2)
Host Guinea Pig
(206), (139), (17), (4)
Clonality
Polyclonal
Conjugate This TGFA antibody is un-conjugated
(26), (13), (10), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(180), (174), (163), (159), (146), (115), (113), (65), (62), (18), (14), (13), (8), (4), (4), (2), (2), (2), (1), (1)
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling Images
Sequence MGHHHHHHSG SEF- ENSTSPL SDPPVAAAVV SHFNDCPDSH TQFCFHGTCR FLVQEDKPAC VCHSGYVGAR CEHADLLAVV AASQKKQA
Specificity It has been selected for its ability to recognize TGFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant TGFa expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against TGFa.
Isotype IgG

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name Transforming Growth Factor Alpha (TGFA Antibody Abstract)
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant TGFa (Glu24~Ala98) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibody (ABIN1860726) anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibody