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|Antigen||Transforming Growth Factor, alpha (TGFA) Antibodies|
|Reactivity||Cow (Bovine), Human, Rat (Rattus) Alternatives|
|Conjugate||This TGFA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
|1 reference available|
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Product Details anti-TGFA AntibodyTarget Details TGFA Application Details Handling References for anti-TGFA antibody (ABIN336238) Images
|Specificity||This antibody Reacts with TGFα and Does not react with EGF or TGFβ. Human uterous, ovary or foreskin.|
|Immunogen||Recombinant Mature human TGFalpha.|
Target Details TGFAProduct Details anti-TGFA Antibody Application Details Handling References for anti-TGFA antibody (ABIN336238) Images back to top
|Alternative Name||TGF-alpha (TGFA) (TGFA Antibody Abstract)|
|Background||Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33 % homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
|Pathways||NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-TGFA Antibody Target Details TGFA Handling References for anti-TGFA antibody (ABIN336238) Images back to top
Immunoprecipitation: This antibody has been used at dilutions from 1/250-1/1000 toimmunoprecipitate mature and higher molecular weight forms of human TGFα from thetissue culture fluid of metabolically labelled cells over-expressing the full length TGFαprecursor molecule as well as from rat and bovine tissue preparations. Immunohistochemistry on Frozen and Paraffin Embedded Tissues: Reacts withparaffin-fixed human uterus, ovary and foreskin at 1/25-1/100. In ELISA with 1 μg/mL synthetic TGFα in the coating buffer the end point dilution was1/16,000. Homologous RIA titres range from 1/30k-1/100k (human and bovine) and1/2k-1/10k (rat) at a sensitivity of 0.2 ng minimum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TGFA Antibody Target Details TGFA Application Details References for anti-TGFA antibody (ABIN336238) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-TGFA antibody (ABIN336238)Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling Images back to top
Ju, Velu, Vass, Papageorge, Lowy: "Tumorigenic transformation of NIH 3T3 cells by the autocrine synthesis of transforming growth factor alpha." in: The New biologist, Vol. 3, Issue 4, pp. 380-8, 1991