anti-Human CHEK1 antibody for Dot Blot

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
pSer317
70
66
39
33
16
15
15
15
14
12
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
486
196
168
14
14
10
10
10
7
5
4
4
3
2
1
1
1
Host
Rabbit
422
55
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
411
178
144
75
69
40
25
21
20
10
7
6
6
5
Options
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Catalog No. ABIN389543
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.80606 ABIN3028564 DB IHC ELISA WB Rabbit Ig Fraction pSer317 Log in to see Polyclonal
1 ABIN931013 DB Mouse IgG1 Log in to see 2178C1a
1 ABIN1956170 DB IHC ELISA WB FITC Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN1958500 DB IHC ELISA WB PE Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN1953727 DB IHC ELISA WB APC Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN1952855 DB IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN1955009 DB IHC ELISA WB Biotin Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN1957386 DB IHC ELISA WB HRP Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN1831864 DB Mouse IgG1 Log in to see 2178C1a
1 ABIN2594884 DB ELISA IHC WB Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN2232853 DB IHC ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN2232855 DB IHC ELISA WB APC Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN2232858 DB IHC ELISA WB HRP Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN2232857 DB IHC ELISA WB FITC Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN2232859 DB IHC ELISA WB PE Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN2232854 DB IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN2232856 DB IHC ELISA WB Biotin Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN1840447 DB IHC (p) WB Rabbit pSer317 Log in to see Polyclonal
1 ABIN2594891 DB WB Mouse IgG1 Log in to see 2178C1a
1 ABIN2794095 DB IHC WB Rabbit Ig Fraction pSer317 Log in to see Polyclonal

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer317
(70), (66), (39), (33), (16), (15), (15), (15), (14), (12), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(486), (196), (168), (14), (14), (10), (10), (10), (7), (5), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(422), (55), (23), (6)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(411), (178), (144), (75), (69), (40), (25), (21), (20), (10), (7), (6), (6), (5)
Pubmed 3 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN389543) Images
Specificity This Phospho-CHK1-S317 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Clone RB7845-RB18425
Isotype Ig

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 antibody (ABIN389543) Images back to top
Antigen
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
Synonyms: Serine/threonine-protein kinase Chk1, CHEK1, CHK1,
Molecular Weight 54420 DA
Gene ID 1111
UniProt O14757
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 antibody (ABIN389543) Images back to top
Application Notes DB = 1:500, WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 antibody (ABIN389543) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CHEK1 antibody (ABIN389543)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Krämer, Mailand, Lukas, Syljuåsen, Wilkinson, Nigg, Bartek, Lukas: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004

Ng, Lee, Ho, Arooz, Siu, Lau, Poon: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN389543) back to top
Supplier Images
Dot Blot (DB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN389543) Dot blot analysis of anti-Phospho-CHK1-S317 Antibody (ABIN389543) on nitrocellulose m...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN389543) The anti-Phospho-CHK1-S317 Pab (ABIN391324) is used in Western blot for detection in,...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN389543) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...