You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CHEK1 antibody for Dot Blot

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
pSer317
57
48
37
34
28
20
14
13
12
10
9
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
451
150
140
5
4
4
3
2
2
2
1
1
Host
Rabbit
393
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
374
180
138
63
60
34
25
21
20
8
6
5
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN389543
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.63784635 ABIN2160084 DB IHC WB Rabbit Ig Fraction pSer317 Log in to see Polyclonal
0.0008934913 ABIN931013 DB Mouse IgG1 Log in to see 2178C1a
0.0008934913 ABIN1952855 DB IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser317 Log in to see Polyclonal
0.0008934913 ABIN1953727 DB IHC ELISA WB APC Rabbit IgG Ser317 Log in to see Polyclonal
0.0008934913 ABIN1956170 DB IHC ELISA WB FITC Rabbit IgG Ser317 Log in to see Polyclonal
0.0008934913 ABIN1955009 DB IHC ELISA WB Biotin Rabbit IgG Ser317 Log in to see Polyclonal
0.0008934913 ABIN1958500 DB IHC ELISA WB PE Rabbit IgG Ser317 Log in to see Polyclonal
0.0008934913 ABIN1957386 DB IHC ELISA WB HRP Rabbit IgG Ser317 Log in to see Polyclonal
0.0008934913 ABIN1831864 DB Mouse IgG1 Log in to see 2178C1a
0.0008934913 ABIN2594884 DB Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN2232857 DB IHC ELISA WB FITC Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN2232855 DB IHC ELISA WB APC Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN2232859 DB IHC ELISA WB PE Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN2232858 DB IHC ELISA WB HRP Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN2232853 DB IHC ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN2232856 DB IHC ELISA WB Biotin Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN2232854 DB IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG pSer317 Log in to see Polyclonal
0.0008934913 ABIN1840447 DB IHC (p) WB Rabbit pSer317 Log in to see Polyclonal
0.0008934913 ABIN2594891 DB WB Mouse IgG1 Log in to see 2178C1a
0.0008934913 ABIN3028564 DB IHC ELISA WB Rabbit Ig Fraction pSer317 Log in to see Polyclonal

Similar anti-CHEK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 22 Antibodies
ELISA 164 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 140 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Western Blotting (WB) 362 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer317
(57), (48), (37), (34), (28), (20), (14), (13), (12), (10), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(451), (150), (140), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(393), (51), (22), (6)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(374), (180), (138), (63), (60), (34), (25), (21), (20), (8), (6), (5), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN389543) Images
Specificity This Phospho-CHK1-S317 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Clone RB7845-RB18425
Isotype Ig

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN389543) Images back to top
Antigen
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
Synonyms: Serine/threonine-protein kinase Chk1, CHEK1, CHK1,
Molecular Weight 54420 DA
Gene ID 1111
UniProt O14757
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN389543) Images back to top
Application Notes DB = 1:500, WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN389543) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CHEK1 Antibody (ABIN389543)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Krämer, Mailand, Lukas, Syljuåsen, Wilkinson, Nigg, Bartek, Lukas: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004

Ng, Lee, Ho, Arooz, Siu, Lau, Poon: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN389543) back to top
Supplier Images
Dot Blot (DB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer317) (ABIN389543) Dot blot analysis of anti-Phospho-CHK1-S317 Antibody (ABIN389543) on nitrocellulose m...
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer317) (ABIN389543) The anti-Phospho-CHK1-S317 Pab (ABIN391324) is used in Western blot for detection in,...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer317) (ABIN389543) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...