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anti-Human CHEK1 antibody for Flow Cytometry

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
C-Term
58
48
37
33
28
20
14
13
12
10
9
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
447
147
137
5
4
4
3
2
2
2
1
1
Host
Rabbit
391
50
21
6
Clonality
Monoclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
371
180
135
61
58
34
23
22
19
8
6
4
1
1
Supplier
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Available images

Catalog No. ABIN189257
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010616893 ABIN2983591 ICC FACS IF WB Rabbit IgG Log in to see Polyclonal

Similar anti-CHEK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 22 Antibodies
ELISA 164 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 140 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Western Blotting (WB) 362 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope C-Term
(58), (48), (37), (33), (28), (20), (14), (13), (12), (10), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(447), (147), (137), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(391), (50), (21), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(371), (180), (135), (61), (58), (34), (23), (22), (19), (8), (6), (4), (1), (1)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen.
Clone EP691Y
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays animportant role in DNA damage checkpoint, embryonic development and tumorsuppression (1). Chk1 is activated in response to replication blocks and genotoxicstress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to,phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25cprevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3,4). Alternate Names: anti-CHEK 1 antibody, anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe)antibody, anti-Cell cycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Chk1antibody, anti-Serine/threonine protein kinase Chk1 antibody, anti-CHEK 1 antibody,anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe) antibody, anti-Cellcycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Serine/threonine proteinkinase Chk1 antibody.
Gene Symbol: CHEK1
Gene ID 1111, 12649, 140583
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 56kDa.
Recommended dilutions: Flow Cytometry 1:160, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Western Blot 1:1000-10000
Protocol Protocol specific for Chk1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (C-Term) (ABIN189257) Western blot analysis on HeLa cell lysate using anti-Chk1 RabMAb (ABIN189257), diluti...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (C-Term) (ABIN189257) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-C...