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|Application / Reactivity||Human|
|Dot Blot (DB)||22 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||6 Antibodies|
|Immunocytochemistry (ICC)||25 Antibodies|
|Immunofluorescence (IF)||61 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies|
|Immunohistochemistry (IHC)||130 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||54 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies|
|Proximity Ligation Assay (PLA)||7 Antibodies|
|Western Blotting (WB)||348 Antibodies|
|Antigen||Checkpoint Kinase 1 (CHEK1) Antibodies|
|Conjugate||This CHEK1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-CHEK1 AntibodyTarget Details CHEK1 Application Details Handling Images
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Immunogen||A synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen.|
Target Details CHEK1Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
|Alternative Name||CHK1 (CHEK1 Antibody Abstract)|
Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays animportant role in DNA damage checkpoint, embryonic development and tumorsuppression (1). Chk1 is activated in response to replication blocks and genotoxicstress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to,phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25cprevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3,4). Alternate Names: anti-CHEK 1 antibody, anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe)antibody, anti-Cell cycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Chk1antibody, anti-Serine/threonine protein kinase Chk1 antibody, anti-CHEK 1 antibody,anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe) antibody, anti-Cellcycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Serine/threonine proteinkinase Chk1 antibody.
Gene Symbol: CHEK1
|Gene ID||1111, 12649, 140583|
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
Application DetailsProduct Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 56kDa.
Recommended dilutions: Flow Cytometry 1:160, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Western Blot 1:1000-10000
Protocol specific for Chk1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top