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anti-Human CHEK1 antibody for Immunoprecipitation

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Reactivity
Human
431
138
129
5
4
3
3
2
2
2
1
1
Host
Mouse
378
46
21
6
Clonality
Monoclonal
Conjugate
This CHEK1 antibody is un-conjugated
12
12
11
8
8
8
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
354
173
125
56
51
34
22
21
17
8
6
4
1
1
Supplier
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Catalog No. ABIN487487
$ 346.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008904695 ABIN264916 IP WB Sheep IgG AA 1-474 Log in to see Polyclonal
0.0008904695 ABIN298041 IP WB Goat IgG Log in to see Polyclonal
0.0008904695 ABIN151777 IP PLA WB Goat Log in to see Polyclonal
0.0008904695 ABIN121782 IP WB Goat Log in to see Polyclonal
0.0008904695 ABIN293715 IP WB Goat Log in to see Polyclonal
0.0008904695 ABIN1977788 IP PLA WB Goat IgG AA 250-300 Log in to see Polyclonal
0.0008904695 ABIN1977789 ICC IF IP IHC (p) PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal
0.0008904695 ABIN323824 IP WB Rabbit AA 250-300 Log in to see Polyclonal
0.0008904695 ABIN2137553 IP WB Mouse IgG1 kappa Log in to see AF7H4
0.0008904695 ABIN1981995 IP WB Sheep IgG AA 157-476 Log in to see Polyclonal
0.0008904695 ABIN300652 IP WB Sheep Log in to see Polyclonal
0.0008904695 ABIN2166872 IP WB Goat IgG Log in to see Polyclonal
0.0008904695 ABIN2472954 IP WB Goat IgG Log in to see Polyclonal
0.0008904695 ABIN323823 IP WB Goat IgG Log in to see Polyclonal
0.0008904695 ABIN2232850 IP WB Goat Log in to see Polyclonal
0.0008904695 ABIN644920 IP WB Goat Log in to see

Similar anti-CHEK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 22 Antibodies
ELISA 158 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 61 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 130 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 54 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Western Blotting (WB) 347 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Reactivity Human
(431), (138), (129), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(378), (46), (21), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHEK1 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(354), (173), (125), (56), (51), (34), (22), (21), (17), (8), (6), (4), (1), (1)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Specificity This antibody reacts with CHK1 (55 kDa) on Western blots.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: CHEK1, CHEK-1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog
Purification Protein-A Sepharose Chromatography.
Immunogen Human recombinant full length CHK1. Remarks: Hybridoma was established by fusion of Mouse myeloma cell NS-2 with Balb/cmouse splenocyte
Clone DCS-310
Isotype IgG2b

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background The DNA damage checkpoint is a signal transduction pathway that delays entry into mitosis following DNA damage. When DNA is damaged, Chk1 acts downstream of ATM to elicit appropriate responses such as cell cycle arrest. When activated by ATM, Chk1 phosphorylates serines 123, 178, 278, and 292 of the S phase-promoting CDC25A phosphatase, which accelerates IR (ionizing radiation)-induced degradation of CDC25A.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
Gene ID 1111
UniProt O14757
Research Area Kinases/Phosphatases, Cell Cycle
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes Western Blot: 1 μg/mL mL for chemiluminescence detection system. Positive Controls: HeLa, MCF7, Raji Cells. Immunoprecipitation: 2 μg/200 μL of cell extract from 5x10^6 cells. Positive Control: Raji. Immunohistochemistry: 1 μg/mLHeat treatment is necessary for Paraffin Embedded Sections. Microwave oven: 2 times for 10 minutes each in citrate buffer ( pH 6.5). Positive Control: Tonsil Tissue. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for the transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggested in the APPLICATIONS for 1 hour at room temperature. (Theoptimal antibody concentration will depend on the experimental conditions. )8) Wash the membrane with PBS (5 minutes x 6 times). 9) Incubate the membrane with the 1: 10000 POD-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriatechemiluminescence reagents for 1 minute. Remove extra reagent from the membrane bydabbing with a paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. Theconditions for exposure and development may vary. Positive Controls for Western blotting: HeLa, MCF7, RajiImmunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add primary antibody as suggested in the APPLICATIONS into 200 μL of the supernatant. Mix well and incubate with gentle agitation for 30-120minutes at 4°C. Add 20 μL of 50% protein A agarose beads resuspended in the cold Lysisbuffer. Mix well and incubate with gentle agitation for 60 minutes at 4°C. 4) Wash the beads 3-5 times with ice-cold Lysis buffer (centrifuge the tube at 2,500 x g for10 seconds). 5) Resuspend the beads in 20 μL of Laemmli’s sample buffer, boil for 3-5 minutes, andcentrifuge for 5 minutes. Use 10 μL/lane for the SDS-PAGE analysis. (See SDS-PAGE & Western blotting. )Positive Controls for immunoprecipitation: Raji.
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 50 % Glycerol without preservatives.
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (ABIN487487) anti-Checkpoint Kinase 1 (CHEK1) antibody
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (ABIN487487) anti-Checkpoint Kinase 1 (CHEK1) antibody (Image 2)
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (ABIN487487) anti-Checkpoint Kinase 1 (CHEK1) antibody (Image 3)